Capture and printing of fixed stromal cell membranes for bioactive display on PDMS surfaces.
Capture and printing of fixed stromal cell membranes for bioactive display on PDMS surfaces.
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捕获和打印固定基质细胞膜，以便在 PDMS 表面上显示生物活性。
DOI：
10.1021/la4012795
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Parekkadan,Biju
中科院分区：
文献类型：
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作者：
Lee,Jungwoo;Wang,JenniferB;Bersani,Francesca;Parekkadan,Biju
聚（二甲基硅氧烷）（PDMS）已成为用于各种生物应用的极其有用的聚合物。将PDMS与生物活性分子缀合以产生功能表面是可行的，但限于单分子显示，分子在PDMS上的定位不精确。在这里，我们报告了一个强大的技术，可以转移和打印的膜表面的戊二醛固定的基质细胞完整的PDMS基板上使用的中间聚乙烯醇（PVA）膜作为转运系统。捕获整个表面分子的细胞-PVA膜可以剥离并随后印刷到PDMS上，同时保持原始细胞表面分子的空间显示。使用人骨髓基质细胞膜描述了概念验证研究，包括证明转移膜捕获和粘附造血细胞的生物活性。所提出的方法适用于几乎任何贴壁细胞，并且可以拓宽生物分子在PDMS上的功能展示，以用于生物技术应用。
Poly(dimethylsiloxane) (PDMS) has emerged as an extremely useful polymer for various biological applications. The conjugation of PDMS with bioactive molecules to create functional surfaces is feasible yet limited to a single-molecule display with imprecise localization of the molecules on PDMS. Here we report a robust technique that can transfer and print the membrane surface of glutaraldehyde-fixed stromal cells intact onto a PDMS substrate using an intermediate polyvinylalcohol (PVA) film as a transporter system. The cell-PVA film capturing the entirety of surface molecules can be peeled off and subsequently printed onto PDMS while maintaining the spatial display of the original cell surface molecules. Proof-of-concept studies are described using human bone marrow stromal cell membranes including a demonstration of the bioactivity of transferred membranes to capture and adhere hematopoietic cells. The presented process is applicable to virtually any adherent cell and can broaden the functional display of biomolecules on PDMS for biotechnology applications.