LEVELS OF 7-ALPHA-HYDROXY-4-CHOLESTEN-3-ONE IN PLASMA REFLECT RATES OF BILE-ACID SYNTHESIS IN MAN
LEVELS OF 7-ALPHA-HYDROXY-4-CHOLESTEN-3-ONE IN PLASMA REFLECT RATES OF BILE-ACID SYNTHESIS IN MAN
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DOI：
10.1016/0014-5793(88)80944-x
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发表时间：
1988-11-07
期刊：
影响因子：
3.5
通讯作者：
SJOVALL, J
中科院分区：
文献类型：
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作者：
AXELSON, M;ALY, A;SJOVALL, J
描述了血浆中 7α-羟基-4-胆固醇-3-酮的分析方法。固相萃取/纯化后，使用紫外检测器通过高效液相色谱法测定化合物。健康受试者的中位浓度为 12 ng/ml（范围 3-40）。在与胆汁酸产生量低相关的疾病中，该水平较低：肝外胆汁淤积，<1.5 ng/ml（范围<0.9-3）；肝硬化 <1.5 ng/ml（范围 < 0.9–38），并且在与高胆汁酸产生相关的疾病中更高：考来烯胺治疗，188 ng/ml（范围 54–477）；回肠切除 397 ng/ml（范围 128–750）。在接受结肠切除术的患者中，该水平基本正常。结果与血浆中 7α-羟基-4-胆固醇-3-酮水平和胆汁酸合成速率之间的强正相关性一致。
A method for analysis of 7α‐hydroxy‐4‐cholesten‐3‐one in plasma is described. Following solid‐phase extraction/purification the compound is determined by high‐performance liquid chromatography using a UV detector. The median concentration in healthy subjects was 12 ng/ml (range 3–40). The levels were lower in diseases associated with a low bile acid production: extrahepatic cholestasis, <1.5 ng/ml (range < 0.9–3); liver cirrhosis <1.5 ng/ml (range < 0.9–38), and higher in diseases associated with a high bile acid production: cholestyramine treatment, 188 ng/ml (range 54–477); ileal resection 397 ng/ml (range 128–750). The levels were essentially normal in patients with colon resection. The results are consistent with a strong positive correlation between the levels of 7α‐hydroxy‐4‐cholesten‐3‐one in plasma and the rate of bile acid synthesis.