A highly selective fluorescence turn-on sensor for Cysteine/Homocysteine and its application in bioimaging
A highly selective fluorescence turn-on sensor for Cysteine/Homocysteine and its application in bioimaging
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DOI：
10.1021/ja073140i
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发表时间：
2007-08-29
影响因子：
15
通讯作者：
Huang, Chunhui
中科院分区：
文献类型：
--
作者：
Zhang, Meng;Yu, Mengxiao;Huang, Chunhui
我们展示了一个高度缺电子系统1作为生物样品中Cys/Hcy细胞内成像的开启荧光传感器。基于Cys和Hcy中巯基的特异性亲核能力，1对Cys/Hcy的选择性和敏感性高于其他氨基酸和巯基生物分子。该探针是第一个在可见光区激发并开启（75倍）荧光发射的Cys/Hcy传感器，并且还显着增加了用于传感Cys/Hcy的双光子激发荧光。此外，共聚焦激光扫描显微镜和双光子激光扫描显微镜实验进一步证实了1可以用于生物样品中Cys/Hcy的传感。
We demonstrated a highly electron-deficient system 1 as a turn-on fluorescence sensor for intracellular imaging of Cys/Hcy in biological samples. On the basis of the specific nucleophilic ability of thiols of Cys and Hcy, 1 exhibited high selectivity and sensitivity for Cys/Hcy over other amino acids and thiol biomolecules. This probe is the first Cys/Hcy sensor with excitation in the visible region and turn-on (75-fold) fluorescence emission and also gives a significant increase in two-photon excited fluorescence for sensing Cys/Hcy. Moreover, confocal laser scanning microscopy and two-photon laser scanning microscopy experiments further established that 1 can be used for sensing Cys/Hcy within biological samples.