Calbindin-immunopositive cells are cholinergic interneurons in the myenteric plexus of rabbit ileum
Calbindin-immunopositive cells are cholinergic interneurons in the myenteric plexus of rabbit ileum
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DOI：
10.1007/s00441-004-0931-5
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发表时间：
2004-11-01
影响因子：
3.6
通讯作者：
Gábriel, R
中科院分区：
文献类型：
--
作者：
Dénes, V;Gábriel, R
28 kDa 钙结合蛋白（calbindin）是许多物种肠神经系统中广泛研究的神经元标记物。先前已在兔回肠的肌间神经元中检测到钙结合蛋白，其中所有肌间神经元的 3% 为钙结合蛋白免疫阳性。我们研究了肠道这一部分钙结合蛋白免疫阳性神经元的详细形态和化学编码。我们在强染色和弱染色的神经元中发现了钙结合蛋白免疫反应性。其中，强免疫反应性神经元属于 Dogiel I 型神经元。这些神经元仅投射到其他神经节和神经丛的初级股，并且它们的过程从未延伸到肌肉或粘膜层。该组内的神经元长度为 29.5+/-6.6 微米，宽度为 14.7+/-3.8 微米。第二小组免疫反应细胞 (27%) 标记微弱，并具有不同的形态特性。它们的特征是圆形中等大小的细胞体（长轴：24.4+/-5.2 mm；短轴：15.5+/-2.9 mm），并且在树突或轴突中不表现出免疫反应性。双标记研究表明，所有钙结合蛋白免疫阳性神经元均缺乏一氧化氮合酶、血管活性肠肽和P物质的免疫反应性，但均对乙酰胆碱合成酶、胆碱乙酰转移酶具有免疫反应性。因此，含有钙结合蛋白的神经元群是兔回肠肌间神经丛中的胆碱能中间神经元。
The 28-kDa calcium-binding protein (calbindin) is a widely studied neuronal marker in the enteric nervous system of numerous species. Calbindin has previously been detected in myenteric neurons of rabbit ileum in which 3% of all myenteric neurons are calbindin-immunopositive. We have studied the detailed morphology and chemical coding of calbindin-immunopositive neurons in this segment of the gut. We have found calbindin immunoreactivity in both strongly and weakly stained neurons. Of these, the strongly immunoreactive neurons belong to the Dogiel type I category. These neurons project only to other ganglia and primary strands of the plexus and their processes never run to the muscle or mucosal layers. The neurons within this group are 29.5+/-6.6 mum in length and 14.7+/-3.8 mum in width. The second smaller group of immunoreactive cells (27%) label faintly and have different morphological properties. They are characterized by their round medium-sized cell bodies (long axis: 24.4+/-5.2 mum; short axis: 15.5+/-2.9 mum) and do not exhibit immunoreactivity either in their dendrites or in their axonal processes. Double-label studies show that all calbindin-immunopositive neurons lack immunoreactivity for nitric oxide synthase, vasoactive intestinal peptide and substance P but all are immunoreactive for the synthesizing enzyme of acetylcholine, choline acetyltransferase. Thus, populations of neurons containing calbindin are cholinergic interneurons in the myenteric plexus of rabbit ileum.