Monitoring systemic infection by cucumber mosaic virus using a small fluorescent protein iLOV in plants
Monitoring systemic infection by cucumber mosaic virus using a small fluorescent protein iLOV in plants
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DOI：
10.1007/s10327-022-01100-6
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发表时间：
2022-10-12
影响因子：
1.2
通讯作者：
Nakahara, Kenji S.
中科院分区：
文献类型：
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作者：
Kawakubo, Ayaka;Gallois, Jean-Luc;Nakahara, Kenji S.
跟踪植物中的病毒感染对评估植物的敏感性或抗性是有用的。表达2b融合绿色荧光蛋白(GFP，25 kDa)的重组黄瓜花叶病毒(CMV)在植物体内逐渐丢失GFP基因。在这里，我们构建了表达基于黄素的小荧光蛋白(Ilov，10 kDa)的CMV。CMV-Ilov在接种烟草的上部叶片中28dpi后仍保留Ilov，并允许对其在植物中的分布进行长期监测。利用CMV-Ilov，我们证明了POT1是一个隐性的抗马铃薯Y病毒的等位基因，在番茄中不具有CMV抗性。
Tracking virus infection in plants is useful to assess plant susceptibility or resistance. The recombinant cucumber mosaic virus (CMV) expressing a 2b-fused green fluorescent protein (GFP, 25 kDa) gradually loses (within weeks) the GFP gene in plants. Here, we constructed CMV expressing a flavin-based small fluorescent protein (iLOV, 10 kDa). CMV-iLOV still retains iLOV after 28 dpi in upper leaves of inoculated Nicotiana benthamiana and allows long-term monitoring of its distribution in plants. Using CMV-iLOV, we showed that pot1, a recessive allele for resistance to potyviruses, does not confer CMV resistance in tomato.