Double Labeling of Microtubules and Actin Filaments in Maize Leaf Division Zone
Double Labeling of Microtubules and Actin Filaments in Maize Leaf Division Zone
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DOI：
10.21769/bioprotoc.3262
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发表时间：
2019
期刊：
影响因子：
0.8
通讯作者：
Qiong Nan;Janette Mendoza;M. Facette
中科院分区：
文献类型：
--
作者：
Qiong Nan;Janette Mendoza;M. Facette
[摘要]玉米叶片呈梯度生长。玉米幼叶底部含有较高比例的对称性和非对称性分裂细胞，是研究细胞分裂的有效模式系统。在这里，我们描述了一种双重染色的方法来显示固定的玉米叶片组织中的肌动蛋白细丝和微管，使用免疫荧光染色微管和荧光阳离子染料染色肌动蛋白细丝。该方法为玉米细胞分裂过程中细胞骨架的研究提供了一种技术手段。
[Abstract] Maize leaves grow in a gradient. The base of young maize leaves contains a high proportion of symmetrically and asymmetrically dividing cells, making the leaf an effective model system for the study of cell division. Here, we describe a double staining method to visualize actin filaments and microtubules in fixed maize leaf tissues, using immunofluorescent staining for microtubules and fluorescent phalloidin staining for actin filaments. This method provides a technique for the study of the cytoskeleton during maize cell division.