Phrenic nerve diabetic neuropathy in rats: unmyelinated fibers morphometry
Phrenic nerve diabetic neuropathy in rats: unmyelinated fibers morphometry
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DOI：
10.1111/j.1529-8027.2009.00223.x
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发表时间：
2009-06-01
影响因子：
3.8
通讯作者：
Moore, Kenneth C.
中科院分区：
文献类型：
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作者：
Fazan, Valeria Paula S.;Rodrigues Filho, Omar A.;Moore, Kenneth C.
我们已经证明，在链脲佐菌素(STZ)诱导的糖尿病大鼠中，膈神经的大有髓纤维表现出轴突萎缩，这一现象可被胰岛素治疗逆转。然而，在STZ神经病模型中，对小的有髓纤维和无髓纤维结构异常的研究有限。此外，神经内血管系统的结构变化在这个模型中没有被清楚地描述，需要详细的研究。我们对胰岛素治疗和未治疗的STZ糖尿病大鼠和非糖尿病对照组动物进行了为期12周的膈神经形态计量学研究。用透射电子显微镜评价神经病变的存在，并对无髓纤维进行形态计量。最引人注目的发现是注射STZ导致的小髓纤维神经病的形态证据，这种疾病不能被传统的胰岛素治疗保护或逆转。除了胰岛素治疗外，这种神经病变明显与两个STZ组的神经内血管严重损伤有关。STZ-糖尿病模型被广泛用于研究实验性糖尿病神经病变，但很少有研究对该动物模型中的无髓纤维或毛细血管形态进行详细评估。本研究为进一步研究糖尿病神经功能的超微结构基础提供了有用的信息。
We have demonstrated that phrenic nerves' large myelinated fibers in streptozotocin (STZ)-induced diabetic rats show axonal atrophy, which is reversed by insulin treatment. However, studies on structural abnormalities of the small myelinated and the unmyelinated fibers in the STZ-model of neuropathy are limited. Also, structural changes in the endoneural vasculature are not clearly described in this model and require detailed study. We have undertaken morphometric studies of the phrenic nerve in insulin-treated and untreated STZ-diabetic rats and non-diabetic control animals over a 12-week period. The presence of neuropathy was assessed by means of transmission electron microscopy, and morphometry of the unmyelinated fibers was performed. The most striking finding was the morphological evidence of small myelinated fiber neuropathy due to the STZ injection, which was not protected or reversed by conventional insulin treatment. This neuropathy was clearly associated with severe damage of the endoneural vessels present on both STZ groups, besides the insulin treatment. The STZ-diabetes model is widely used to investigate experimental diabetic neuropathies, but few studies have performed a detailed assessment of either unmyelinated fibers or capillary morphology in this animal model. The present study adds useful information for further investigations on the ultrastructural basis of nerve function in diabetes.