Luteinizing hormone (LH)-releasing hormone in third ventricular cerebrospinal fluid of the ewe: correlation with LH pulses and the LH surge.
Luteinizing hormone (LH)-releasing hormone in third ventricular cerebrospinal fluid of the ewe: correlation with LH pulses and the LH surge.
复制标题
母羊第三脑室脑脊液中的黄体生成素 (LH) 释放激素：与 LH 脉冲和 LH 浪涌的相关性。
作者：
D. C. Skinner;B. Malpaux;B. Delaleu;A. Caraty
形态学证据表明，LHRH可以分泌到脑脊液中，但只有在恒河猴的脑脊液中才发现LHRH反映了LHRH神经内分泌系统的变化。这项研究调查了绵羊脑脊液中是否可以检测到LHRH，如果是的话，它的释放谱是否与脉冲和电涌条件下的外周黄体生成素谱相关。将聚乙烯导管穿过预先植入去卵巢母羊第三脑室的不锈钢导尿管，使其能够连续多次抽出脑脊液。第一个实验(n=3)表明，以12、30、50和100微升/分钟的速度去除脑脊液对外周血中的黄体生成素浓度没有影响。第二个实验(n=4)证实了脑脊液LHRH的分泌是脉动性的，脉冲幅度有很大的变化(6.3+/-1.8pg/ml；范围1.3-18.7pg/ml)。在第三个实验中(n=6)，停用黄体酮和注射17β-雌二醇后产生内源性黄体生成素高峰。虽然脑脊液LHRH(15.3+/-1.3h)和外周血LHRH(14.8+/-1.0h)同时出现，但脑脊液LHRH浓度(11.0+/-0.6h；P<；0.005)大于黄体生成素浓度(6.0h/-0.4h)。这是首次对绵羊进行的研究揭示了脑脊液中LHRH的存在，并表明它表现出与外周促黄体生成素波动一致的动态和长期变化。脑脊液促性腺激素释放激素分析还允许对个体进行重复采样，从而进行长期的个体内比较。
Morphological evidence suggests that LHRH may be secreted into cerebrospinal fluid (CSF), but only in the rhesus monkey has CSF LHRH been found to reflect changes occurring in the LHRH neuroendocrine system. This study investigated whether LHRH is detectable in ovine CSF and, if so, whether its release profile correlates with peripheral LH profiles during pulse and surge conditions. A polyethylene catheter was threaded through a stainless steel guide cannula previously implanted into the third ventricle of an ovariectomized ewe, which enabled continuous CSF withdrawal on repeated occasions. The first experiment (n = 3) showed that peripheral LH concentrations were unaffected by CSF removal at rates of 12, 30, 50, and 100 microliters/min, and the second (n = 4) established that CSF LHRH secretion was pulsatile, with considerable variation in pulse amplitude (6.3 +/- 1.8 pg/ml; range, 1.3-18.7 pg/ml). In the third experiment (n = 6), an endogenous LH surge was induced after progesterone withdrawal and 17 beta-estradiol administration. Although CSF LHRH (15.3 +/- 1.3 h) and peripheral LH (14.8 +/- 1.0 h) surges occurred simultaneously, CSF LHRH concentrations were greater than half-maximal levels for longer (11.0 +/- 0.6 h; P < 0.005) than LH concentrations (6.0 +/- 0.4 h). This is the first study in sheep to reveal the presence of LHRH in CSF and show that it expresses dynamic and longer term changes coincident with peripheral LH fluctuations. CSF LHRH analysis also permits repeated sampling from individuals and, therefore, long term within-individual comparisons.
在恒河猴中，促性腺激素释放激素的激增伴随着雌二醇诱导的促性腺激素的激增。
影响因子：
4.8
作者：
Xia,L;VanVugt,D;Alston,EJ;Luckhaus,J;Ferin,M
通讯作者：
Ferin,M
抗 GnRH 血清和酚妥拉明对 GnRH 脉冲的干扰会消除去卵巢兔子的搏动性 LH 分泌，但不会消除 FSH 分泌。
影响因子：
4.1
作者：
Pau,KY;Gliessman,PM;Oyama,T;Spies,HG
通讯作者：
Spies,HG