FLOW-INJECTION ANALYSIS WITH ENZYME THERMISTOR DETECTOR FOR AUTOMATED-DETERMINATION OF BETA-LACTAMS
FLOW-INJECTION ANALYSIS WITH ENZYME THERMISTOR DETECTOR FOR AUTOMATED-DETERMINATION OF BETA-LACTAMS
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DOI：
10.1021/ac00266a032
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发表时间：
1984-01-01
影响因子：
7.4
通讯作者：
DANIELSSON, B
中科院分区：
文献类型：
--
作者：
DECRISTOFORO, G;DANIELSSON, B
开发了一种新的自动化流动注射分析（FIA）方法，用于快速和特异性地测定β-内酰胺，例如，青霉素和头孢菌素。在连续流动的流中，用热敏电阻测量酶促反应的速率。酶反应在小型固体床反应器柱上进行，由此对各种分析物的选择性完全取决于所选酶系统的特异性。通过与高效液相色谱法的比较，评价了酶热敏电阻FIA（ET-FIA）的准确性。两种方法之间在统计学上无显著差异。ET-FIA可检出的青霉素V最小量为10-6 g。通过连续进样10次相同样品测定方法的精密度;相对标准偏差为±.百分之零点八。青霉素和头孢菌素在0.1-10 mg/ml浓度范围内呈线性。样品容量为40-45个样品/h;单个样品的分析时间< 80 s。
A new, automated flow injection analysis (FIA) method was developed for rapid and specific determination of .beta.-lactams, e.g., penicillins and cephalosporins. The enthalpies of enzymatic reactions were measured in continuous flowing streams by thermistors. Enzyme reactions were carried out on small solid bed reactor columns, whereby the selectivity for various analytes was exclusively dependent on the specificity of the chosen enzyme system. Accuracy of enzyme thermistor FIA (ET-FIA) was evaluated by comparison with high performance liquid chromatography. Statistically no significant difference was found between the 2 methods. The minimal amount of K-penicillin V detectable by ET-FIA was 10-6 g. Precision of the method was determined by 10 consecutive injections of the same sample; the relative standard deviation was .+-. 0.8%. Linearity was obtained in a concentration range of 0.1-10 mg/ml for penicillins and cephalosporins. Sample capacity was 40-45 samples/h; analysis time for 1 single sample was < 80 s.