Molecular characterization of five betacryptoviruses infecting four clover species and dill
Molecular characterization of five betacryptoviruses infecting four clover species and dill
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DOI：
10.1007/s00705-013-1691-x
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发表时间：
2013-09-01
影响因子：
2.7
通讯作者：
Maiss, Edgar
中科院分区：
文献类型：
--
作者：
Lesker, Till;Rabenstein, Frank;Maiss, Edgar
部分病毒科包括具有双节段dsRNA基因组和等轴病毒体的植物（Alphacryptovirus和Betacryptovirus）、真菌（Partitivirus）和原生动物（Cryspovirus）病毒。隐病毒通常发生在不同的植物物种中，不会引起任何症状。到目前为止，已经确定了许多序列的病毒属的甲纹病毒，但没有序列是可用于任何指定的成员属的倍纹病毒。在本研究中，提取，克隆和双链RNA的序列分析，我们报告了三个指定的成员Betacryptovirus属，白色三叶草隐病毒2，红色三叶草隐病毒2和啤酒花三叶草隐病毒2，和两个新的推定Betacryptovirus中发现的深红色三叶草和莳萝的分子特性。Betacryptoviruses与Partitivirus属的成员共享序列基序。在系统发育分析中，Betacryptovirus属的成员在包含Partitivirus属成员的簇内形成了一个新的亚簇。我们的研究结果为植物和真菌dsRNA病毒的不同进化谱系提供了证据。
The family Partitiviridae includes plant (Alphacryptovirus and Betacryptovirus), fungal (Partitivirus) and protozoan (Cryspovirus) viruses with bisegmented dsRNA genomes and isometric virions. Cryptic viruses commonly occur in different plant species without causing any symptoms. So far, numerous sequences have been determined for viruses of the genus Alphacryptovirus, but no sequence is available for any assigned member of the genus Betacryptovirus. Following extraction, cloning and sequence analysis of double-stranded RNA in this study, we report the molecular properties of three assigned members of the genus Betacryptovirus, white clover cryptic virus 2, red clover cryptic virus 2 and hop trefoil cryptic virus 2, and two new putative betacryptoviruses found in crimson clover and dill. Betacryptoviruses share sequence motifs with members of the genus Partitivirus. In phylogenetic analyses, members of the genus Betacryptovirus formed a new sub-cluster within the clusters containing members of the genus Partitivirus. Our results provide evidence for a distinct evolutionary lineage of dsRNA viruses of plants and fungi.