MOLECULAR-CLONING OF OLFACTOMEDIN, AN EXTRACELLULAR-MATRIX PROTEIN-SPECIFIC TO OLFACTORY NEUROEPITHELIUM
MOLECULAR-CLONING OF OLFACTOMEDIN, AN EXTRACELLULAR-MATRIX PROTEIN-SPECIFIC TO OLFACTORY NEUROEPITHELIUM
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DOI：
10.1073/pnas.90.10.4655
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发表时间：
1993-05-15
影响因子：
11.1
通讯作者：
ANHOLT, RRH
中科院分区：
文献类型：
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作者：
YOKOE, H;ANHOLT, RRH
嗅觉神经上皮细胞外粘液基质是一种高度组织化的结构，与容纳嗅觉转导机制的化学感应纤毛密切接触。在这里，我们描述了 olfactomedin 的分子克隆和一级结构，它是这种细胞外基质的主要成分。 Olfactomedin 仅在嗅神经上皮中表达，其氨基酸序列与任何已知蛋白质没有同源性。这种嗅觉组织特异性糖蛋白含有半胱氨酸，半胱氨酸形成二硫键连接的聚合物，构成嗅觉细胞外基质的主要结构。与神经系统的其他细胞外基质蛋白类似，嗅觉素可能影响嗅觉神经元顶端树突上化学感应纤毛的维持、生长或分化。
The extracellular mucous matrix of olfactory neuroepithelium is a highly organized structure in intimate contact with chemosensory cilia that house the olfactory transduction machinery. Here we describe the molecular cloning and primary structure of olfactomedin, which is the major component of this extracellular matrix. Olfactomedin is expressed exclusively in olfactory neuroepithelium and its amino acid sequence shows no homologies to any known protein. This olfactory tissue-specific glycoprotein contains cysteines which form disulfide-linked polymers that constitute the primary architecture of the olfactory extracellular matrix. By analogy to other extracellular matrix proteins of the nervous system, olfactomedin may influence the maintenance, growth, or differentiation of chemosensory cilia on the apical dendrites of olfactory neurons.