GLUCOCORTICOIDS AND REGULATION OF PHOSPHOENOLPYRUVATE CARBOXYKINASE ACTIVITY IN RAT BROWN ADIPOSE-TISSUE
GLUCOCORTICOIDS AND REGULATION OF PHOSPHOENOLPYRUVATE CARBOXYKINASE ACTIVITY IN RAT BROWN ADIPOSE-TISSUE
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DOI：
10.1152/ajpendo.1978.235.2.e197
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发表时间：
1978-01-01
影响因子：
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通讯作者：
HIRST, M
中科院分区：
文献类型：
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作者：
FELDMAN, D;HIRST, M
与白色脂肪组织（WAT）进行比较，已知其中[磷酸烯醇式丙酮酸羧激酶]（PEPCK）活性受糖皮质激素调节。大鼠棕色脂肪组织（BAT）中的PEPCK活性对肾上腺切除术或地塞米松没有反应，而WAT活性分别增加和减少。发现BAT PEPCK活性被刺激的三种情况：禁食（1），喂养高脂肪/低碳水化合物饮食（2）和新生儿期。在每种情况下，糖皮质激素治疗阻止了PEPCK活性的刺激，并将酶恢复到基线水平。在条件1和2中，WAT中的酶活性也被刺激，但与BAT相比，糖皮质激素给药将活性降低至显著低于基线活性的低水平。发现了两种抑制BAT中PEPCK活性的条件：暴露于寒冷环境和饲喂高蛋白/低脂肪饮食。WAT PEPCK不因暴露于寒冷而改变。BAT和WAT之间PEPCK调节的差异得到证实，BAT对糖皮质激素的反应是独特的。
Comparison was made to white adipose tissue (WAT) where [phosphoenolpyruvate carboxykinase] (PEPCK) activity is known to be glucocorticoid regulated. PEPCK activity in rat brown adipose tissue (BAT) did not respond to adrenalectomy or dexamethasone, whereas WAT activity was increased and decreased, respectively, by these maneuvers. Three conditions were found in which BAT PEPCK activity was stimulated: fasting (1), feeding a high fat/low carbohydrate diet (2) and during the neonatal period. In each case glucocorticoid treatment prevented the stimulation in PEPCK activity and restored the enzyme to base-line levels. In conditions 1 and 2, enzyme activity was also stimulated in WAT, but in contradistinction to BAT, glucocorticoid administration reduced activity to low levels significantly below base-line activity. Two conditions were found which suppressed PEPCK activity in BAT: exposure to a cold environment and feeding a high protein/low fat diet. WAT PEPCK was unaltered by exposure to cold. Differences in PEPCK regulation between BAT and WAT were demonstrated and the response to glucocorticoids was unique in BAT.