ELECTROPHORETIC MOBILITY AND HYDROPHOBICITY AS A MEASURE TO PREDICT THE INITIAL STEPS OF BACTERIAL ADHESION

ELECTROPHORETIC MOBILITY AND HYDROPHOBICITY AS A MEASURE TO PREDICT THE INITIAL STEPS OF BACTERIAL ADHESION
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DOI:
10.1128/aem.53.8.1898-1901.1987
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发表时间:
1987-08-01
影响因子:
4.4
通讯作者:
ZEHNDER, AJB
ZEHNDER, AJB
中科院分区:
生物学2区
文献类型:
--
作者:
VANLOOSDRECHT, MCM;LYKLEMA, J;ZEHNDER, AJB

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研究了表面物理化学参数与细菌细胞与带负电荷聚苯乙烯的粘附性之间的关系。分别用接触角测量法和电泳法测定细胞表面疏水性和动电电位。这两个参数都会影响细胞的黏附。动电势的影响随着疏水性的降低而增大。决定粘附性的细胞表面特征受生长条件的影响。在高速生长的情况下,细菌细胞往往变得更加疏水性。这一事实对于控制细菌在整个环境中的传播具有生态意义。
The relationship between physiochemical surface parameters and adhesion of bacterial cells to negatively charged polystyrene was studied. Cell surface hydrophobicity and electrokinetic potential were determined by contact angle measurement and electrophoresis, respectively. Both parameters influence cell adhesion. The effect of the electrokinetic potential increases with decreasing hydrophobicity. Cell surface characteristics determining adhesion are influenced by growth conditions. At high growth rates, bacterial cells tend to become more hydrophobic. This fact can be of ecological significance for controlling the spread of bacteria throughout the environment.