Development of an artificial calcium-dependent transcription factor to detect sustained intracellular calcium elevation.
Development of an artificial calcium-dependent transcription factor to detect sustained intracellular calcium elevation.
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DOI：
10.1021/sb500070c
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发表时间：
2014-10
影响因子：
4.7
通讯作者：
Shingo Suzuki;K. Murotomi;Y. Nakajima;K. Kawai;Ken-ichi Ohta;K. Warita;T. Miki;Y. Takeuchi
中科院分区：
文献类型：
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作者：
Shingo Suzuki;K. Murotomi;Y. Nakajima;K. Kawai;Ken-ichi Ohta;K. Warita;T. Miki;Y. Takeuchi
对细胞内钙信号作出反应的合成转录因子的开发，使得在单细胞水平上分析细胞事件或“重新布线”细胞内信息网络成为可能。在这项研究中，我们开发了钙依赖性转录因子（CaTF），它被calpain切割，然后转移到细胞核中，在那里它诱导报告基因的表达。我们的研究结果表明，catf介导的报告基因表达是稳定的，并对细胞内钙水平和钙蛋白酶活性有反应。此外，CaTF检测到表皮生长因子（EGF）生理刺激诱导的持续钙升高。这些结果表明CaTF可能是分析细胞内钙信号的有用工具，并且是内源性信号网络和合成基因网络之间的接口。
The development of a synthetic transcription factor that responds to intracellular calcium signals enables analyzing cellular events at the single-cell level or "rewiring" the intracellular information networks. In this study, we developed the calcium-dependent transcription factor (CaTF), which was cleaved by calpain and then translocated to the nuclei where it induced reporter expression. Our results demonstrated that CaTF-mediated reporter expression was stable and responded to the intracellular calcium level and calpain activity. In addition, CaTF detected the sustained calcium increase that was induced by physiological stimulation with epidermal growth factor (EGF). These results suggest that CaTF could be a useful tool to analyze intracellular calcium signals and be an interface between an endogenous signal network and synthetic gene network.