Effects of phenobarbital and interleukin-6 on cytochrome P4502B1 and 2B2 in cultured rat hepatocytes.
Effects of phenobarbital and interleukin-6 on cytochrome P4502B1 and 2B2 in cultured rat hepatocytes.
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苯巴比妥和白细胞介素 6 对培养大鼠肝细胞中细胞色素 P4502B1 和 2B2 的影响。
DOI：
10.1016/s0006-2952(95)02391-7
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发表时间：
1996
影响因子：
5.8
通讯作者：
Wecker,L
中科院分区：
文献类型：
--
作者：
Clark,MA;Williams,JF;Gottschall,PE;Wecker,L
The objectives of this study were to characterize further the effects of phenobarbital (PB) on cytochrome P4502B1 and 2B2 (P4502B1/2) enzyme activity and immunoreactivity in rat hepatocytes and to investigate the mechanism(s) mediating the ability of interleukin-6 (IL-6) to inhibit this induction. PB caused a concentration-dependent increase in benzyloxyresorufin O-deethylase (BROD) activity with maximal effects (a 25-fold increase) at concentrations of 0.3 to 1 mM. The induction of BROD activity was linear over 24 hr of exposure. Immunoblot profiles of P4502B1/2 agreed with measurements of enzyme activity. In addition to inducing P4502B1/2, PB (0.75 mM) also increased the levels of P450 reductase by approximately 2-fold following a 24-hr exposure to PB. When IL-6 was added concomitantly with or up to 12 hr after the addition of PB, the PB induction of BROD activity and immunoreactivity was inhibited significantly. When 18 hr elapsed between the time of addition of PB and IL-6, the inhibitory effects of IL-6 were no longer apparent, suggesting that the actions of IL-6 were mediated by early events in the induction process. IL-6 did not affect the PB induction of P450 reductase. To determine whether IL-6 altered the degradation of P4502B1/2, hepatocytes were exposed to PB for 24 hr, then washed, and the loss of BROD activity and immunoreactivity following incubation with a protein synthesis inhibitor was measured. IL-6 did not alter the rate of loss of either enzyme activity or immunoreactivity, indicating that the effects of IL-6 could not be attributed to the enhanced degradation of P4502B1/2. Results suggest that the inhibition of PB-induced BROD activity by IL-6 is due to an action on early cellular and molecular events in the induction process.
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影响因子：
3.6
作者：
E. Morgan
通讯作者：
E. Morgan
细菌内毒素诱导的急性期反应期间小鼠肝细胞色素 P-450 同工酶的增强和抑制。
DOI：
--
发表时间：
1988
期刊：
European Journal of Biochemistry
影响因子：
--
作者：
L. Stanley;D. Adams;R. Lindsay;R. Meehan;W. Liao;C. Wolf
通讯作者：
C. Wolf
IL-1 α、IL-6 和地塞米松对细胞色素 P450IIC12 表达的调节。
影响因子：
3.6
作者：
Wright,K;Morgan,ET
通讯作者：
Morgan,ET
DOI：
10.1042/bj2710113
发表时间：
1990
期刊：
The Biochemical journal
影响因子：
--
作者：
Waxman,DJ;Morrissey,JJ;Naik,S;Jauregui,HO
通讯作者：
Jauregui,HO
黄嘌呤氧化酶在干扰素引起的细胞色素 P-450 损失中的作用。
影响因子：
2.1
作者：
S. Moochhala;K. Renton
通讯作者：
K. Renton