Kid-mediated chromosome compaction ensures proper nuclear envelope formation
Kid-mediated chromosome compaction ensures proper nuclear envelope formation
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DOI：
10.1016/j.cell.2008.01.029
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发表时间：
2008-03-07
期刊：
影响因子：
64.5
通讯作者：
Yamamoto, Tadashi
中科院分区：
文献类型：
--
作者：
Ohsugi, Miho;Adachi, Kenjiro;Yamamoto, Tadashi
在有丝分裂末期，相邻的染色体紧密地聚集在一起，形成一个紧密的簇。这对于在整个染色体团周围而不是单个染色体周围重新组装核被膜是重要的。通过分析小鼠和培养的细胞缺乏表达的chromokinesin Kid/kinesin-10，我们发现，Kid定位于后期和末期染色体的边界，并有助于缩短后期染色体质量沿着纺锤体轴。Kid介导的后期染色体致密化的丧失通常导致多核细胞的形成，特别是在卵母细胞减数分裂II和导致胚胎死亡的第一对有丝分裂。与此相反，无论是男性减数分裂或桑椹胚阶段后的体细胞有丝分裂是受Kid缺乏症。这些数据表明，基德介导的后期/末期染色体压实防止形成多核细胞。这种保护在发育的早期阶段尤其重要，此时胚胎细胞富含卵质。
Toward the end of mitosis, neighboring chromosomes gather closely to form a compact cluster. This is important for reassembling the nuclear envelope around the entire chromosome mass but not individual chromosomes. By analyzing mice and cultured cells lacking the expression of chromokinesin Kid/kinesin-10, we show that Kid localizes to the boundaries of anaphase and telophase chromosomes and contributes to the shortening of the anaphase chromosome mass along the spindle axis. Loss of Kid-mediated anaphase chromosome compaction often causes the formation of multinucleated cells, specifically at oocyte meiosis II and the first couple of mitoses leading to embryonic death. In contrast, neither male meiosis nor somatic mitosis after the morula-stage is affected by Kid deficiency. These data suggest that Kid-mediated anaphase/telophase chromosome compaction prevents formation of multinucleated cells. This protection is especially important during the very early stages of development, when the embryonic cells are rich in ooplasm.