Frequency of Cry1F Non-Recessive Resistance Alleles in North Carolina Field Populations of Spodoptera frugiperda (Lepidoptera: Noctuidae)
Frequency of Cry1F Non-Recessive Resistance Alleles in North Carolina Field Populations of Spodoptera frugiperda (Lepidoptera: Noctuidae)
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DOI：
10.1371/journal.pone.0154492
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发表时间：
2016-04-27
期刊：
影响因子：
3.7
通讯作者：
Feng, Hongqiang
中科院分区：
文献类型：
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作者：
Li, Guoping;Reisig, Dominic;Feng, Hongqiang
秋粘虫 Spodoptera frugiperda (J.E. Smith)（鳞翅目：夜蛾科）是表达单一和金字塔状苏云金芽孢杆菌 (Bt) 毒素的转基因玉米 (Zea mays L.) 的目标物种。 2014年，从北卡罗来纳州的光诱捕器中收集了草地贪夜蛾，并筛选了总共212个草地贪夜蛾F-1/F-2同雌性系的Bt和非Bt玉米抗性。所有 212 个品系均对表达 Cry1A.105 + Cry2Ab、Cry1F + Cry1A.105 + Cry2Ab 和 Cry1F + Cry1Ab + Vip3Aa20 的玉米组织敏感。进行生长速率生物测定以分离非隐性 Bt 抗性等位基因。 212 个同雌系中的 7 个个体携带主要的非隐性等位基因，赋予对 Cry1F 的抗性。由这七个个体创建了一个混合群体。该菌落对 Cry1F 的抵抗力是已知易感群体的 151.21 倍，并且对 Cry1A.105 也具有抵抗力，但对 Cry2Ab 和 Vip3Aa20 没有抵抗力。结果表明，从北卡罗来纳州收集的草地贪夜蛾种群普遍对 Cry1F 敏感，但有些个体携带抗性等位基因。本研究生成的数据可用作耐药性监测的基线数据。
Fall armyworm, Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae), is a target species of transgenic corn (Zea mays L.) that expresses single and pyramided Bacillus thuringiensis (Bt) toxin. In 2014, S. frugiperda were collected from a light trap in North Carolina, and a total of 212 F-1/F-2 isofemale lines of S. frugiperda were screened for resistance to Bt and non-Bt corn. All of the 212 isolines were susceptible to corn tissue expressing Cry1A.105 + Cry2Ab, Cry1F + Cry1A.105 + Cry2Ab, and Cry1F + Cry1Ab + Vip3Aa20. Growth rate bioassays were performed to isolate non-recessive Bt resistance alleles. Seven individuals out of the 212 isofemale lines carried major non-recessive alleles conferring resistance to Cry1F. A pooled colony was created from the seven individuals. This colony was 151.21 times more resistant to Cry1F than a known-susceptible population and was also resistant to Cry1A.105, but was not resistant to Cry2Ab and Vip3Aa20. The results demonstrate that field populations of S. frugiperda collected from North Carolina are generally susceptible to Cry1F, but that some individuals carry resistant alleles. The data generated in this study can be used as baseline data for resistance monitoring.