Crypt cell development in newborn rat small intestine.
Crypt cell development in newborn rat small intestine.
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DOI：
10.1083/jcb.100.5.1601
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发表时间：
1985-05
期刊：
影响因子：
--
通讯作者：
Quaroni A
中科院分区：
文献类型：
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作者：
Quaroni A
用3日龄大鼠小肠细胞膜（YBB 1/27，YBB 3/10）和成年大鼠隐窝细胞膜（CC 4/80）制备了3株单克隆抗体。这些抗体被证明可以确定肠隐窝细胞发育的特定阶段。YBB 1/27抗原于妊娠20天在胎儿小肠上皮细胞的腔膜上首次被检测到，出生后1 - 20- 22天仅限于隐窝细胞和下绒毛细胞，而在老年动物的任何肠区域都不能检测到。YBB 3/10抗原被鉴定为一组高Mr蛋白，出生后定位于胎儿肠细胞和隐窝和绒毛细胞的整个表面膜上;断奶后（出生后20-22天），它逐渐从绒毛细胞中消失，并局限于隐窝区域。CC 4/80抗原是一种分子量为28-34 kD的蛋白质（或一组相关蛋白质），在出生后10-14 d出现在腺细胞中。断奶后，其分布发生变化，从腺窝消失，定位于绒毛下部的吸收细胞。这种模式的变化可能部分是由于在年轻动物中注射可的松而过早引起的。这些结果证明了肠腺细胞上存在特定的表面膜成分，并表明胎儿抗原可能在出生后保留在这些细胞中。
Three monoclonal antibodies were prepared against luminal membranes from small intestinal cells of 3-d-old rats (YBB 1/27, YBB 3/10) and crypt cell membranes from adult rats (CC 4/80). The antibodies were shown to define specific stages of development of the intestinal crypt cells. The YBB 1/27 antigen was first detected at the luminal membrane of the epithelial cells in fetal intestine at day 20 of gestation; it was confined to the crypt cells and lower villus cells between 1 and 20- 22 d after birth, and could not be detected in any region of the intestine in older animals. The YBB 3/10 antigen, identified as a set of high Mr proteins, was localized over the entire surface membrane of fetal intestinal cells and of crypt and villus cells after birth; after weaning (20-22 d after birth) it gradually disappeared from the villus cells and became confined to the region of the crypts. The CC 4/80 antigen, identified as a protein (or a set of related proteins) of molecular mass 28-34 kD, was shown to appear in the crypt cells 10-14 d after birth. Its distribution changed after weaning, when it disappeared from the crypts, and was localized in the absorptive lower villus cells. This change in pattern could, in part, be prematurely elicited by cortisone injection in younger animals. These results have demonstrated the presence of specific surface membrane components on the intestinal crypt cells, and suggested that fetal antigens may be retained in these cells after birth.