REQUIREMENT OF TYROSINE PHOSPHORYLATION FOR RAPID ACTIVATION OF A DNA-BINDING FACTOR BY IL-4
REQUIREMENT OF TYROSINE PHOSPHORYLATION FOR RAPID ACTIVATION OF A DNA-BINDING FACTOR BY IL-4
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DOI：
10.1126/science.7694370
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发表时间：
1993-11-19
期刊：
影响因子：
56.9
通讯作者：
REICH, NC
中科院分区：
文献类型：
--
作者：
KOTANIDES, H;REICH, NC
白细胞介素-4（IL-4）是由活化的T淋巴细胞产生的一种免疫调节细胞因子，用于促进参与免疫防御的细胞的生长和分化。本研究证明了IL-4对特异性DNA结合因子的快速激活。IL-4核活化因子（IL-4 NAF）在IL-4刺激后几分钟内出现，并识别IL-4应答基因启动子中发现的特异性DNA序列。这种假定的转录因子的激活需要酪氨酸磷酸化，并且磷酸酪氨酸特异性抗体识别IL-4 NAF-DNA复合物。因此，IL-4似乎通过潜在DNA结合因子的酪氨酸磷酸化将信号传递到细胞核。
Interleukin-4 (IL-4) is an immunoregulatory cytokine produced by activated T lymphocytes to promote the growth and differentiation of cells that participate in immune defense. This study demonstrates the rapid activation of a specific DNA binding factor by IL-4. The IL-4 nuclear-activated factor (IL-4 NAF) appeared within minutes of IL-4 stimulation and recognized a specific DNA sequence found in the promoters of IL-4-responsive genes. Activation of this putative transcription factor required tyrosine phosphorylation, and antibodies specific for phosphotyrosine recognize the IL-4 NAF-DNA complex. Thus, IL-4 appears to transduce a signal to the nucleus through tyrosine phosphorylation of a latent DNA binding factor.