Detection and identification of benzo[a]pyrene diol epoxide adducts to DNA utilizing capillary eleotrophoresis eleotrospray mass spectrometry
Detection and identification of benzo[a]pyrene diol epoxide adducts to DNA utilizing capillary eleotrophoresis eleotrospray mass spectrometry
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DOI：
10.1021/ac9510245
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发表时间：
1996-04-15
影响因子：
7.4
通讯作者：
Vouros, P
中科院分区：
文献类型：
--
作者：
Barry, JP;Norwood, C;Vouros, P
采用毛细管区带电泳（CZE）-负离子电喷雾质谱（ES-MS）联用技术，对苯并[a]芘（BaP）的活性代谢产物（+/-）-反-7，8，9，10-四氢苯并[a]芘-7，8-二醇-9，10-环氧化合物（BPDE）与DNA反应生成的加合物进行了检测和鉴定。结果表明，本文提出的低纳克检测限（
Capillary zone electrophoresis (CZE) coupled with negative ion electrospray mass spectrometry (ES-MS) is used for the detection and identification of adducts formed from the reaction of DNA with (+/-)-anti-7,8,9,10-tetrahydrobenzo[a]pyrene-7,8-diol 9,10-epoxide (BPDE), an active metabolite of benzo[a]pyrene (BaP). Results presented in this paper demonstrate low nanogram detection limits (