The GCR1 requirement for yeast glycolytic gene expression is suppressed by dominant mutations in the SGC1 gene, which encodes a novel basic-helix-loop-helix protein.
The GCR1 requirement for yeast glycolytic gene expression is suppressed by dominant mutations in the SGC1 gene, which encodes a novel basic-helix-loop-helix protein.
复制标题
酵母糖酵解基因表达的 GCR1 需求受到 SGC1 基因显性突变的抑制，该基因编码一种新型碱性螺旋-环-螺旋蛋白。
DOI：
10.1128/mcb.15.5.2646
复制
发表时间：
1995
影响因子：
5.3
通讯作者：
Holland,MJ
中科院分区：
文献类型：
--
作者：
Nishi,K;Park,CS;Pepper,AE;Eichinger,G;Innis,MA;Holland,MJ
TheGCR1gene product is required for maximal transcription of yeast glycolytic genes and for growth of yeast strains in media containing glucose as a carbon source. Dominant mutations in two genes,SGC1andSGC2, as well as recessive mutations in theSGC5gene were identified as suppressors of the growth and transcriptional defects caused by agcr1null mutation. The wild-type and mutant alleles ofSGC1were cloned and sequenced. The predicted amino acid sequence of theSGC1gene product includes a region with substantial similarity to the basic-helix-loop-helix domain of the Myc family of DNA-binding proteins. TheSGC1-1dominant mutant allele contained a substitution of glutamine for a highly conserved glutamic acid residue within the putative basic DNA binding domain. A second dominant mutant,SGC1-2, contained a valine-for-isoleucine substitution within the putative loop region. TheSGC1-1dominant mutant suppressed theGCR1requirement for enolase, glyceraldehyde-3-phosphate dehydrogenase, phosphoglycerate kinase, phosphoglyc-erate mutase, and pyruvate kinase gene expression. Expression of the yeast enolase genes was reduced three-to fivefold in strains carrying ansgc1null mutation, demonstrating thatSGC1is required for maximal enolase gene expression. Expression of the enolase genes in strains carryinggcr1andsgc1double null mutations was substantially less than observed for strains carrying either null mutation alone, suggesting thatGCR1andSGC1function on parallel pathways to activate yeast glycolytic gene expression.
