Effect of oxidized fish oil, vitamin E and ethoxyquin on the histopathology and haematology of rainbow trout, Salmo gairdneri Richardson
Effect of oxidized fish oil, vitamin E and ethoxyquin on the histopathology and haematology of rainbow trout, Salmo gairdneri Richardson
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氧化鱼油、维生素 E 和乙氧基喹啉对虹鳟组织病理学和血液学的影响
DOI：
10.1111/j.1365-2761.1984.tb00932.x
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
H. Ferguson
中科院分区：
文献类型：
--
作者：
R. Moccia;S. Hung;S. Slinger;H. Ferguson
． 在实际饲料中添加不同浓度的氧化鱼油，并添加或不添加维生素E和乙氧基醌，对虹鳟鱼进行24周的喂养。研究了血清生化、血液学和组织病理学，以测定维生素E （DL - α -生育酚乙酸酯）和/或合成替代品（乙氧基醌）对过氧化值分别为120或314mEq/kg油的高度或极度氧化油的相对保护作用。在不添加DL - α -生育酚醋酸酯和添加或不添加乙氧喹啉的情况下，饲喂高度或极度氧化油的组表现出红细胞数量、血红蛋白含量、红细胞压积和溶血增加。此外，这些组的鱼增加，异常发育的多色细胞，脾脏血色素沉着症和肝蜡样病。结果表明，与乙氧喹啉相比，维生素E在较低浓度下具有更好的保护作用，当饲粮中添加7.5%的高氧化油时，每kg添加33 mg DL - α -生育酚乙酸酯足以预防维生素E缺乏症。在含有高氧化油的饲料中单独添加乙氧喹啉似乎能发挥部分保护作用，但与DL - α -生育酚醋酸酯同时添加时不能提高保护水平。这些数据支持维生素E具有一般抗氧化功能的理论，并表明维生素E缺乏导致的死亡是由于肝功能障碍和贫血的累积影响。
. Rainbow trout were fed for 24 weeks on practical diets containing varying concentrations of oxidized fish oil, with or without supplementation of vitamin E and ethoxyquin. Serum biochemistry, haematology and histopathology were investigated to measure the relative protection offered by vitamin E (DL–α–toco–pherol acetate) and/or a synthetic substitute (ethoxyquin), against highly or extremely oxidized oil with peroxide values of 120 or 314mEq/kg oil respectively. Groups fed highly or extremely oxidized oils without DL–α–tocopherol acetate supplementation, and with or without supplemental ethoxyquin, exhibited lower red blood cell numbers, haemoglobin content, haematocrit, and increased haemolysis. In addition, fish from these groups had increased, abnormally developing polychromatocytes, splenic haemosiderosis and hepatic ceroidosis. The results indicate that vitamin E protects better and at lower concentrations than does ethoxyquin, and that supplementation with 33 mg of DL–α–tocopherol acetate/kg is adequate to prevent vitamin E deficiency signs when feeding practical diets containing 7.5 % of a highly oxidized oil. Supplementation with ethoxyquin alone to diets containing highly oxidized oil appeared to exert partial protection, but did not increase the level of protection when added simultaneously with DL–α–tocopherol acetate. The data support the theory of a general antioxidant function for vitamin E, and suggest that mortalities ensuing from vitamin E deficiency are due to the cumulative effects of liver dysfunction and anaemia.