Identification and evaluation of intervarietal substitution lines of rapeseed (Brassica napus L.) with donor segments affecting the diploidization rate of isolated microspores
Identification and evaluation of intervarietal substitution lines of rapeseed (Brassica napus L.) with donor segments affecting the diploidization rate of isolated microspores
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DOI：
10.1007/s10681-016-1656-8
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发表时间：
2016-02
期刊：
影响因子：
1.9
通讯作者：
Anthimos Kampouridis;Katharina Ziese-Kubon;Nurhasanah;W. Ecke
中科院分区：
文献类型：
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作者：
Anthimos Kampouridis;Katharina Ziese-Kubon;Nurhasanah;W. Ecke
植物种小孢子双单倍体是育种和研究的重要工具。产生双单倍体系的限制性步骤是小孢子的二倍体化。在不同基因型之间的二倍体化率有很大的差异，但对这些差异的遗传控制知之甚少。为了确定携带控制油菜游离小孢子二倍化率的遗传因子的基因组区域，比较了二倍体和单倍体小孢子胚分离群体与“Express 617”和“RS 239”杂交后代BC 1的标记分离。图谱构建后，从同一杂交中选择了10个品种间代换系，其供体片段覆盖了5个基因组区域，这些基因组区域在3个群体中显示出偏斜标记分离的模式，表明控制二倍体化率的基因分离。10个品系的小孢子二倍化率为23.9 ~ 58.7%，而轮回亲本“快617”的二倍化率为52.5%。三个品系的二倍体化率显著低于“Express 617”（P = 0.05）。通过比较显着和非显着品系之间的供体片段，鉴定了覆盖油菜籽基因组的4.17%和6.16%之间的7个基因组区域，其可能包含控制油菜籽中二倍体化速率的遗传因子。此外，还发现了一个标记物，它很有可能与这种因素有关。这些有意义的品系为进一步深入研究这一性状提供了理想的材料。
InBrassicaspecies microspore derived doubled haploid lines are an important tool in breeding and research. A limiting step in the production of doubled haploid lines is the diploidization of the microspores. Strong differences have been observed in diploidization rates between different genotypes but little is know about the genetic control of these differences. To identify genomic regions that carry genetic factors controlling the diploidization rate of isolated microspores of rapeseed, marker segregations were compared between segregating populations of diploid and haploid microspore derived embryos and a BC1from a cross between ‘Express 617’ and ‘RS239’. After map construction ten intervarietal substitution lines from the same cross were selected with donor segments covering five genomic regions that showed a pattern of skewed marker segregations across the three populations indicative of the segregation of genes controlling the diploidization rates. The diploidization rates of microspores of the ten lines ranged from 23.9 to 58.7 % while the recurrent parent ‘Express 617’ showed a rate of 52.5 %. For three lines the diploidization rates were significantly lower (P = 0.05) than the rate of ‘Express 617’. By comparing donor segments between the significant and the non-significant lines, seven genomic regions that cover just between 4.17 and 6.16 % of the rapeseed genome were identified that may contain genetic factors controlling diploidization rates in rapeseed. In addition, one marker was found that has a high probability to be linked to such a factor. The significant lines represent an ideal material for further in depth studies of this trait.