Tomato plant leaves: From by-products to the management of enzymes in chronic diseases
Tomato plant leaves: From by-products to the management of enzymes in chronic diseases
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DOI：
10.1016/j.indcrop.2016.09.036
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发表时间：
2016-12-30
影响因子：
5.9
通讯作者：
Andrade, Paula B.
中科院分区：
文献类型：
--
作者：
Figueiredo-Gonzalez, Maria;Valentao, Patricia;Andrade, Paula B.
番茄是世界上最重要的作物之一，其大量的副产品，如叶子，是生物活性化合物的极好来源，在几个行业中具有潜在的应用。在这项工作中，从番茄植物叶提取物（栽培品种：Caramba，Valentine，Negro，Abuela，Rio Alto，Anairis，Rosa和Yack）首次进行了检测，以建立其植物化学指纹图谱和抑制阿尔茨海默病（AD）关键酶的能力（乙酰胆碱酯酶（AChE）、丁酰胆碱酯酶（BuChE）和5-脂氧合酶（LOX））和糖尿病（DM）中的（α-葡糖苷酶和α-淀粉酶）。水甲醇提取物显示出含有大量的酚类（3774-9252 μ g g g（-1）干提取物）、槲皮素-3-0-芸香糖苷、槲皮素-3-0-戊糖基-芸香糖苷以及绿原酸和新绿原酸，它们是主要的。还分析了富含色素即叶绿素的丙酮提取物（46.40 +/- 3.81-136.4 +/- 7.94 mg g（-1）干提取物）以及富含番茄碱的生物碱提取物（224 +/- 4.39-745 +/- 4.96 mg g（-1）干提取物）。此外，水甲醇提取物对乙酰胆碱酯酶（AChE）具有抑制活性（IC 50 = 3806 +/- 113-9911 +/- 50.5 μ g mL（-1）），BuChE（IC 50 = 133.1 +/- 2.68-369.1 +/- 17.2 μ g mL（-1）），LOX（IC 25 = 35.2 +/- 0.49-533 +/- 8.9 μ g mL（-1）），α-葡糖苷酶（IC 50 = 1.14 +/- 0.04-6.48 +/- 0.13 mg mL（-1））和淀粉酶（IC 50 = 1.11 +/- 0.02-1.78 +/- 0.03 mg mL（-1））。相反，生物碱提取物仅对BuChE有效（IC 50 = 82.4 +/- 1.34-172 +/- 10.2 μ g mL（-1））。化学指纹图谱和生物活性因品种而异。总的来说，我们的研究结果表明，L。番茄叶是用于AD和DM管理的有前景的副产物和有价值的生物活性化合物来源。(C)© 2016 Elsevier B. V.版权所有。
Lycopersicon esculentum Mill. is one of the world's most important crops and its enormous by-products, such as leaves, are an excellent source of bioactive compounds with potential application in several industries. In this work, extracts from tomato plant leaves (cultivars: Caramba, Valentine, Negro, Abuela, Rio Alto, Anairis, Rosa and Yack) were examined, for the first time, to establish their phytochemical fingerprint and capacity to inhibit key enzymes involved in Alzheimeris disease (AD) (acetylcholinesterase (AChE), butyrylcholinesterase (BuChE) and 5-lipoxygenase (LOX)) and in diabetes mellitus (DM) (alpha-glucosidase and alpha-amylase). The hydromethanol extracts were shown to contain high amounts of phenolics (3774-9252 mu g g(-1) of dry extract), quercetin-3-0-rutinoside, quercetin-3-0-pentosyl-rutinoside, and chlorogenic and neochlorogenic acids being the major ones. The acetone extracts rich in pigments, namely chlorophylls (46.40 +/- 3.81-136.4 +/- 7.94 mg g(-1) of dry extract), as well as alkaloids extracts rich in tomatine (224 +/- 4.39-745 +/- 4.96 mg g(-1) of dry extract), were also analysed. Moreover, hydromethanol extracts exhibited inhibitory activity against AChE (IC50 = 3806 +/- 113-9911 +/- 50.5 mu g mL(-1)), BuChE (IC50 = 133.1 +/- 2.68-369.1 +/- 17.2 mu g mL(-1)), LOX (IC25 = 35.2 +/- 0.49-533 +/- 8.9 mu g mL(-1)), alpha-glucosidase (IC50 = 1.14 +/- 0.04-6.48 +/- 0.13 mg mL(-1)) and a amylase (IC50 = 1.11 +/- 0.02-1.78 +/- 0.03 mg mL(-1)). On the contrary, the alkaloid extracts were only effective against BuChE (IC50 = 82.4 +/- 1.34-172 +/- 10.2 mu g mL(-1)). Chemical fingerprint and biological activities varied according to the analysed cultivar. Overall, our results suggest that L. esculentum leaves are promising by-products and valuable sources of bioactive compounds for AD and DM management. (C) 2016 Elsevier B.V. All rights reserved.