Purification and Partial Identification of Bacteriocin ISK-1, a New Lantibiotic Produced by Pediococcus sp. ISK-1.
Purification and Partial Identification of Bacteriocin ISK-1, a New Lantibiotic Produced by Pediococcus sp. ISK-1.
复制标题
片球菌产生的新型羊毛硫抗生素细菌素 ISK-1 的纯化和部分鉴定。
DOI：
10.1271/bbb.62.2341
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
A. Ishizaki
中科院分区：
文献类型：
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作者：
H. Kimura;H. Matsusaki;T. Sashihara;K. Sonomoto;A. Ishizaki
细菌素ISK-1是一种蛋白质抑制物质，由从成熟的鹿鹿子中分离出来的Pediococcus sp. ISK-1产生。采用酸处理、硫酸铵沉淀法、阳离子交换色谱法和反相高效液相色谱法从Pediococcus sp. ISK-1培养上清液中纯化细菌素ISK-1。纯化后的细菌素风险-1比活性提高了30倍，回收率为17%。快速原子轰击-质谱法测定细菌素ISK-1的分子质量为2960。细菌素ISK-1的氨基酸组成分析表明，它含有罕见的氨基酸，如衣硫氨酸和/或3-甲基衣硫氨酸，这是l抗生素的特征。n端氨基酸序列分析表明，前7个n端氨基酸残基为NH2-K-K-K-S-G-V-I。该序列与乳酸乳球菌（Lactococcus lactiin） 481和变异微球菌（Micrococcus varians）的variacin具有显著的相似性，提示细菌素ISK-1是一种新型lantibiotic，属于lactoin -481型。
Bacteriocin ISK-1 is a proteinaceous inhibitory substance produced by Pediococcus sp. ISK-1 isolated from well-aged Nukadoko. Bacteriocin ISK-1 was purified by acid treatment, ammonium sulfate precipitation, cation-exchange chromatography, and reversed-phase HPLC from the culture supernatant of Pediococcus sp. ISK-1. Purification of bacteriocin ISK-1 resulted in a 30-fold increase in the specific activity and the recovery was 17%. Molecular mass of bacteriocin ISK-1 measured by fast atom bombardment-mass spectrometry was 2,960. The amino acid composition analysis of bacteriocin ISK-1 showed that it contained unusual amino acids such as lanthionine and/or 3-methyllanthionine, which is a characteristic of lantibiotics. The N-terminal amino acid sequence analysis indicated the first seven N-terminal amino acid residues as NH2-K-K-K-S-G-V-I. The primary sequence showed significant similarity to the lantibiotics lacticin 481 from Lactococcus lactis and variacin from Micrococcus varians, which suggests that bacteriocin ISK-1 is a novel lantibiotic belonging to a lacticin-481 type.