Ex Vivo Differentiation of Resting CD4+ T Lymphocytes Enhances Detection of Replication Competent HIV-1 in Viral Outgrowth Assays.
Ex Vivo Differentiation of Resting CD4+ T Lymphocytes Enhances Detection of Replication Competent HIV-1 in Viral Outgrowth Assays.
复制标题
静息 CD4 T 淋巴细胞的体外分化增强了病毒生长测定中对具有复制能力的 HIV-1 的检测。
DOI：
10.1007/978-1-0716-1871-4_21
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Kulpa,DeannaA
中科院分区：
文献类型：
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作者：
Wonderlich,ElizabethR;Reece,MonicaD;Kulpa,DeannaA
量化携带可诱导和可复制HIV-1前病毒的细胞数量对于评估HIV-1治疗干预至关重要，但已证明精确量化潜伏库在技术上具有挑战性。现有的方案来量化来自长期ART治疗个体的静息CD4 + T细胞中有复制能力的HIV-1的频率，这有助于研究储库稳定性的动态，然而这些方法对有效评估完整储库所需的HIV-1表达的诱导具有显著的障碍。CD4 + T细胞分化为效应记忆表型是促进体外潜伏期逆转的成功策略，并显著增强了病毒生长测定的性能和灵敏度。
Quantifying the number of cells harboring inducible and replication competent HIV-1 provirus is critical to evaluating HIV-1 cure interventions, but precise quantification of the latent reservoir has proven to be technically challenging. Existing protocols to quantify the frequency of replication-competent HIV-1 in resting CD4+ T cells from long-term ART treated individuals have helped to investigate the dynamics of reservoir stability, however these approaches have significant barriers to the induction of HIV-1 expression required to effectively evaluate the intact reservoir. Differentiation of CD4+ T cells to an effector memory phenotype is a successful strategy for promoting latency reversal in vitro, and significantly enhances the performance and sensitivity of viral outgrowth assays.