Melatonin regulation of antioxidant enzyme gene expression
Melatonin regulation of antioxidant enzyme gene expression
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DOI：
10.1007/pl00012498
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发表时间：
2002-10-01
影响因子：
8
通讯作者：
Rodriguez, C
中科院分区：
文献类型：
--
作者：
Mayo, JC;Sainz, RM;Rodriguez, C
抗氧化酶（AOEs）是抵抗毒素诱导的自由基和/或细胞内自发形成的自由基的主要细胞防御的一部分。褪黑素（Melatonin， MLT）因其直接清除自由基和抗氧化的特性，近年来受到广泛关注。在本研究中，我们报道了在生理血清浓度（约为1 nM）下，MLT增加了两种神经细胞系中超氧化物歧化酶（sod）和谷胱甘肽过氧化物酶（GPx）的mRNA。MLT对sod和GPx mRNA的影响是由一个从头合成的蛋白介导的。MLT改变Cu-Zn SOD和GPx mRNA的稳定性。短时间处理（脉冲作用）MLT的实验表明，AOE基因表达的调节可能是受体介导的，因为MLT处理1小时的反应与处理24小时的反应相同。
Antioxidant enzymes (AOEs) are part of the primary cellular defense against free radicals induced by toxins and/or spontaneously formed in cells. Melatonin (MLT) has received much attention in recent years due to its direct free radical scavenging and antioxidant properties. In the present work we report that MLT, at physiological serum concentrations (approximate to1 nM), increases the mRNA of both superoxide dismutases (SODs) and glutathione peroxidase (GPx) in two neuronal cell lines. The MLT effect on both SODs and GPx mRNA was mediated by a de novo synthesized protein. MLT alters mRNA stability for Cu-Zn SOD and GPx. Experiments with a short time treatment (pulse action) of MLT suggest that the regulation of AOE gene expression is likely to be receptor mediated, because 1-h treatment with MLT results in the same response as a 24-h treatment.