DNA Nanodevices as Mechanical Probes of Protein Structure and Function
DNA Nanodevices as Mechanical Probes of Protein Structure and Function
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DOI：
10.3390/app11062802
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发表时间：
2021-03
期刊：
影响因子：
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通讯作者：
N. Stephanopoulos;P. Šulc
中科院分区：
文献类型：
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作者：
N. Stephanopoulos;P. Šulc
DNA纳米技术已经报道了广泛的结构可调的支架，其尺寸，形状和机械性能具有精确的控制。这些纳米器件的一个有前景的应用是作为蛋白质功能或蛋白质结构测定的探针。在这个角度来看，我们涵盖了最近在这一领域的几个例子，包括确定配体间距和多价性对细胞活化的影响，在纳米级施加力，并帮助解决蛋白质结构的冷冻EM。我们还强调了将蛋白质与DNA纳米支架整合所需的化学方向，以及混合蛋白质-DNA纳米材料的计算建模的机会。
DNA nanotechnology has reported a wide range of structurally tunable scaffolds with precise control over their size, shape and mechanical properties. One promising application of these nanodevices is as probes for protein function or determination of protein structure. In this perspective we cover several recent examples in this field, including determining the effect of ligand spacing and multivalency on cell activation, applying forces at the nanoscale, and helping to solve protein structure by cryo-EM. We also highlight some future directions in the chemistry necessary for integrating proteins with DNA nanoscaffolds, as well as opportunities for computational modeling of hybrid protein-DNA nanomaterials.