A Combined Fluorescence and Microcomputed Tomography System for Small Animal Imaging
A Combined Fluorescence and Microcomputed Tomography System for Small Animal Imaging
复制标题
用于小动物成像的荧光和微计算机断层扫描组合系统
DOI：
10.1109/tbme.2010.2073468
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发表时间：
2010-12-01
影响因子：
4.6
通讯作者：
Bai, Jing
中科院分区：
文献类型：
--
作者：
Guo, Xiaolian;Liu, Xin;Bai, Jing
荧光分子断层成像（FMT）在从分子水平研究小动物体内生理和病理过程中发挥着重要作用。然而，这种技术具有相对低的空间分辨率。为了补充这个问题，同时提供功能和形态分析的需求一直很强烈。在本文中，我们提出了一个混合全角度自由空间FMT和X射线微锥束计算机断层扫描（CT）（微型CBCT）的原型系统，提供功能和解剖图像。在整个采集过程中，两个子系统同步采集投影图像（荧光和CT），以保持一致的体位，而不移动动物。所采集的数据集在相应的坐标和识别的几何结构中本质上是共配准的。分别使用基于标准化Born的空间正则化和Feldkamp-Davis-Kress方法重建断层荧光和CT图像。体模和在体小鼠的实验结果初步验证了该集成系统的准确性和性能。
Fluorescence molecular tomography (FMT) plays an important role in studying physiological and pathological processes of small animals in vivo at molecular level. However, this technique suffers from relatively low spatial resolution. To complement the problem, there has been a strong demand for providing functional and morphological analysis at the same time. In this paper, we proposed a hybrid full-angle free-space FMT and X-ray micro-cone-beam computed tomography (CT) (micro-CBCT) prototype system, providing both functional and anatomical images. During the whole acquisition, the two subsystems acquire projection images (fluorescence and CT) synchronously to keep consistent body position without moving the animals. The acquired datasets are intrinsically coregistered in the corresponding coordinate and identified geometry. Tomographic fluorescence and CT images are reconstructed using normalized Born-based spatial regularization and Feldkamp-Davis-Kress methods, respectively. The experimental results of both phantom and in vivo mouse preliminarily validate the accuracy and performance of the integrated system.