Yeast centrin Cdc31 is linked to the nuclear mRNA export machinery
Yeast centrin Cdc31 is linked to the nuclear mRNA export machinery
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DOI：
10.1038/ncb1163
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发表时间：
2004-09-01
影响因子：
21.3
通讯作者：
Hurt, E
中科院分区：
文献类型：
--
作者：
Fischer, T;Rodríguez-Navarro, S;Hurt, E
中心体蛋白是类钙调蛋白，在微管组织中心的复制中发挥作用。在此我们描述了酵母中心体蛋白Cdc31的一种新功能。我们发现，核输出因子Sac3羧基末端结构域中一个被称为CID的序列过量表达会结合Cdc31，导致显性致死表型以及纺锤体极体（SPB）复制受阻。在正常条件下，CID基序将Cdc31和Sus1（SAGA转录复合物的一个亚基）招募到Sac3 - Thp1复合物中，该复合物与核篮处的特定核孔蛋白一起在mRNA输出中发挥作用。一个先前报道的cdc31温度敏感等位基因，其在SPB复制和Kic1激酶激活方面均无缺陷，但会诱导mRNA输出缺陷。因此，Cdc31与mRNA输出机制存在一种意想不到的联系。
Centrins are calmodulin-like proteins that function in the duplication of microtubule-organizing centres. Here we describe a new function of the yeast centrin Cdc31. We show that overproduction of a sequence, termed CID, in the carboxy-terminal domain of the nuclear export factor Sac3 titrates Cdc31, causing a dominant-lethal phenotype and a block in spindle pole body (SPB) duplication. Under normal conditions, the CID motif recruits Cdc31 and Sus1 (a subunit of the SAGA transcription complex) to the Sac3-Thp1 complex, which functions in mRNA export together with specific nucleoporins at the nuclear basket. A previously reported cdc31 temperature-sensitive allele, which is neither defective in SPB duplication nor Kic1 kinase activation, induces mRNA export defects. Thus, Cdc31 has an unexpected link to the mRNA export machinery.