Role of Liver NK Cells and Peritoneal Macrophages in Gamma Interferon and Interleukin-10 Production in Experimental Bacterial Peritonitis in Mice
Role of Liver NK Cells and Peritoneal Macrophages in Gamma Interferon and Interleukin-10 Production in Experimental Bacterial Peritonitis in Mice
复制标题
肝脏 NK 细胞和腹膜巨噬细胞在小鼠实验性细菌性腹膜炎中产生 γ 干扰素和白细胞介素 10 的作用
作者：
S. Seki;S. Osada;S. Ono;S. Aosasa;Y. Habu;T. Nishikage;H. Mochizuki;H. Hiraide
采用盲肠结扎和18号针穿刺BALB/c小鼠，检测实验性细菌性腹膜炎小鼠肝、脾、肺、外周血单个核细胞（MNC）和腹膜渗出细胞（PEC）产生γ干扰素（IFN-γ）、肿瘤坏死因子α (TNF-α)和白细胞介素10 （IL-10）的情况。培养MNC 18 h，检测上清液中细胞因子水平。腹腔灌洗液中含有的细胞因子被认为是PEC产生的细胞因子。只有肝脏MNC和PEC产生了大量的IFN-γ， PEC是IL-10的主要来源，特别是在CLP后12小时。肝脏MNC和PEC产生的细胞因子反映，CLP后血清IFN-γ和IL-10水平升高。C57BL/6 （B6）小鼠和BALB/c裸小鼠的细胞因子产生模式相似。与假手术小鼠相比，培养上清、腹腔灌洗液和血清中TNF-α水平无显著升高。用抗亚洲蓟GM1或抗nk1.1抗体消耗B6小鼠NK细胞，可显著降低肝脏MNC培养上清和血清中IFN-γ的水平，提示肝脏NK细胞是IFN-γ的产生者。另一方面，塑料粘附的PEC巨噬细胞是IL-10的主要产生者。接受盲肠结扎和切割手术（腹膜炎更严重）的小鼠比接受CLP治疗的小鼠显示出更高的IFN-γ和IL-10水平，而用较小（22号）针接受CLP治疗的小鼠显示出较低的这些细胞因子水平。这些研究结果表明，肝脏NK细胞和PEC巨噬细胞在细菌性腹膜炎的促炎和抗炎细胞因子的产生中起重要作用。
ABSTRACT Gamma interferon (IFN-γ), tumor necrosis factor alpha (TNF-α), and interleukin-10 (IL-10) production by liver, spleen, lung, peripheral blood mononuclear cells (MNC), and peritoneal exudate cells (PEC) in experimental bacterial peritonitis was examined by cecum ligation and puncture (CLP) (with an 18-gauge needle) of BALB/c mice. MNC of organs were cultured for 18 h, and cytokine levels in supernatants were examined. Cytokines contained in peritoneal lavage fluid were regarded as those produced by PEC. Only liver MNC and PEC produced substantial amounts of IFN-γ, and PEC were the main source of IL-10, especially 12 h after CLP. As reflected by the cytokine production by liver MNC and PEC, serum IFN-γ and IL-10 levels were elevated after CLP. C57BL/6 (B6) mice and BALB/c nude mice showed a similar pattern of cytokine production. TNF-α levels in culture supernatants, peritoneal lavage fluid, and sera were not significantly elevated compared to those of sham-operated mice. In vivo depletion of NK cells of B6 mice with anti-asialo GM1 or anti-NK1.1 antibody greatly decreased IFN-γ levels in liver MNC culture supernatants and sera, suggesting that liver NK cells are IFN-γ producers. On the other hand, plastic-adherent PEC macrophages are the major IL-10 producers. Mice subjected to a cecum ligation and cut procedure (which have a more severe peritonitis) showed much higher IFN-γ and IL-10 levels than those subjected to CLP, while mice subjected to CLP with a smaller (22-gauge) needle showed low levels of these cytokines. These findings show that liver NK cells and PEC macrophages are important for the production of proinflammatory and anti-inflammatory cytokines in bacterial peritonitis.
影响因子：
4.4
作者：
Mark K. Eskandari;G. Bolgos;Christopher J. Miller;D. Nguyen;L. Deforge;D. Remick
通讯作者：
Mark K. Eskandari;G. Bolgos;Christopher J. Miller;D. Nguyen;L. Deforge;D. Remick
影响因子：
15.9
作者：
SMEDLY, LA;TONNESEN, MG;WORTHEN, GS
通讯作者：
WORTHEN, GS
内源性 IL-10 可保护小鼠在脓毒性腹膜炎期间免于死亡。
DOI：
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发表时间：
1995
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
vanderPoll,T;Marchant,A;Buurman,WA;Berman,L;Keogh,CV;Lazarus,DD;Nguyen,L;Goldman,M;Moldawer,LL;Lowry,SF
通讯作者：
Lowry,SF