13C nuclear magnetic resonance study of glycogen resynthesis in muscle after glycogen-depleting exercise in healthy men receiving an infusion of lipid emulsion
13C nuclear magnetic resonance study of glycogen resynthesis in muscle after glycogen-depleting exercise in healthy men receiving an infusion of lipid emulsion
复制标题
DOI：
10.2337/diabetes.48.2.327
复制
发表时间：
1999-02-01
期刊：
影响因子：
7.7
通讯作者：
Gin, H
中科院分区：
文献类型：
--
作者：
Delmas-Beauvieux, MC;Quesson, B;Gin, H
在健康个体中，已知在强烈消耗后的糖原恢复在初始阶段是快速的且不依赖于胰岛素，并且随后是缓慢的且依赖于胰岛素。游离脂肪酸（FFA）作为胰岛素抵抗（IR）的一个假定来源，因此可能会损害糖原恢复在第二个时期。使用体内C-13核磁共振（NMR），我们研究了长链甘油三酯乳剂对空腹健康男性（n = 8）进行90分钟中度运动（包括跖屈）后3小时恢复期内腓肠肌糖原再合成的影响。在单独的实验中，每例受试者输注10%意维利匹（0.015 ml.kg（-1）.min（-1））或10%甘油（0.13 mg.kg（-1）.min（-1））。在运动之前和结束时以及恢复期间获得NMR光谱。测定全身葡萄糖和脂质氧化率（间接量热法）、血浆胰岛素、C肽、葡萄糖、乳酸盐、β-羟基丁酸盐、甘油三酯和游离脂肪酸。糖原消耗为初始糖原的47.6 +/- 4.5%（甘油）和49.7 +/- 4.8%（意维利匹）。通过稳态模型评估，意维利匹组的获得性IR在恢复期开始时具有显著性（P = 0.002）。糖原再合成在甘油组出现在第一个小时比在随后的第2个小时的postexercise期间。在恢复期，意维利匹组的糖原再合成水平显著低于甘油组（第1 h P = 0.04，接下来2 h P = 0.001）。在恢复期间，两组的血浆乳酸和全身氧化速率相似，而意维利匹组的血浆乳酸和全身氧化速率显著较高。一个减少细胞摄取葡萄糖作为底物的糖原合成，而不是之间的竞争氧化的碳水化合物和游离脂肪酸，进行了讨论。
In healthy individuals, glycogen recovery after a strong depletion is known to be rapid and insulin independent during the initial phase, and subsequently, slow and insulin dependent. Free fatty acids (FFAs) as a putative source of insulin resistance (IR) could thus impair glycogen recovery during the second period. Using in vivo C-13 nuclear magnetic resonance (NMR), we studied the effect of long-chain triglyceride emulsion on gastrocnemius glycogen resynthesis during a 3-h recovery period after 90 min of moderate exercise consisting of plantar flexion on overnight-fasted healthy men (n = 8). In separate experiments, each subject was infused with 10% Ivelip (0.015 ml.kg(-1).min(-1)) or 10% glycerol (0.13 mg.kg(-1).min(-1)). NMR spectra were acquired before and at the end of the exercise and during the recovery period. Whole-body glucose and lipid oxidation rates (indirect calorimetry), plasma insulin, C-peptide, glucose, lactate, beta-hydroxybutyrate, triglycerides, and FFAs were determined. Glycogen consumption was 47.6 +/- 4.5% (glycerol) and 49.7 +/- 4.8% (Ivelip) of the initial glycogen. An acquired IR in the Ivelip group was significant at the onset of the recovery period by homeostasis model assessment (P = 0.002). Glycogen resynthesis in the glycerol group appeared faster during the Ist h than during the subsequent 2nd h of the postexercise period. The glycogen resynthesis level was significantly lower in the Ivelip group than in the glycerol group during the recovery period (P = 0.04 during the Ist h and P = 0.001 during the next 2 h). During the recovery, plasma lactate and whole-body oxidation rates were similar in the two groups, whereas glycemia was significantly higher in the Ivelip group. A decreased cellular uptake of glucose as a substrate for glycogenosynthesis, rather than a competition between oxidation of carbohydrate and FFA, is discussed.