Cytokinins differentially affect regeneration, plant growth and antioxidative enzymes activity in chive (Allium schoenoprasum L.)
Cytokinins differentially affect regeneration, plant growth and antioxidative enzymes activity in chive (Allium schoenoprasum L.)
复制标题
细胞分裂素对韭菜 (Allium schoenoprasum L.) 的再生、植物生长和抗氧化酶活性有不同的影响
DOI：
10.1007/s11240-015-0869-1
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发表时间：
2015
影响因子：
3
通讯作者：
S. Zdravković
中科院分区：
文献类型：
--
作者：
L. Tubić;J. Savić;N. Mitić;J. Milojević;D. Janošević;S. Budimir;S. Zdravković
与大蒜和洋葱不同，韭菜（Allium schoenoprasumL.）的再生，出于烹饪和观赏目的而种植，尚未得到深入研究。研究了8种细胞分裂素和植株基部厚度对再生效率和植株生长的影响。使用0、1、5或10 μM的所有细胞分裂素结构基团的代表：类异戊二烯侧链（反式玉米素）和芳香族侧链（苄腺嘌呤、激动素、间托泊林）腺嘌呤衍生物和苯基脲衍生物（噻苯隆和N-（2-氯-4-吡啶基）-N′-苯基脲）。组织学分析表明，不定芽从叶鞘基部和基盘形成。以10 μM噻苯隆（TDZ）处理的再生频率最高（100%），平均每个外植体的芽数最多（20.0个），5 mm厚的外植体再生效果最好。通过在4周芽诱导期后用5 μM激动素（Kinetin，Kin）替代或排除10 μM TDZ，避免了通过该处理再生的植株的较差的芽和根生长特性，而不影响再生效率。此外，过氧化物酶、过氧化氢酶和超氧化物歧化酶活性与再生能力呈正相关。10 μM TDZ处理的再生效果最差，而1 μM Kin处理的再生效果最差。此外，一个独特的过氧化物酶亚型，观察到只有TDZ处理的外植体后第3天的治疗。这一工作对于基因工程和脱毒植物生产的发展，以及关于抗氧化酶在植物器官发生中的作用的知识扩展是有用的。
Unlike garlic and onion, the regeneration of chive (Allium schoenoprasumL.), cultivated both for culinary and ornamental purposes, has not been intensively studied. The effects of the eight cytokinins and the plant basal section thickness on regeneration efficiency and subsequent plant growth were studied. Representatives of all cytokinin structural groups: isoprenoide side chain (trans-zeatin) and aromatic side chain (benziladenine, kinetin,meta-topolin) adenine derivatives, and phenylurea derivatives (thidiazuron and N-(2-chloro-4-pyridyl)-N′-phenylurea) at 0, 1, 5 or 10 μM were used. Histological analysis revealed adventitious buds formation from the leaf sheaths’ bases and the basal plate. The highest regeneration frequency (100 %) and the mean bud number per explant (20.0) were achieved with 10 μM thidiazuron (TDZ), and 5 mm-thick basal sections were the most responsive explants. Inferior shoot and root growth characteristics of plants regenerated by this treatment was avoided by exclusion or replacement of 10 μM TDZ with 5 μM kinetin (Kin) after a 4-week bud induction period, without consequences on the regeneration efficiency. In addition, a positive correlation between peroxidase, catalase and superoxide dismutase activity and the regeneration capacity was observed. All antioxidative enzymes activity changed much faster with 10 μM TDZ than with 1 μM Kin, which provoked the weakest regeneration response. Moreover, a unique peroxidase isoform was observed only in TDZ-treated explants after 3rd day of treatment. This work is useful for genetic engineering and virus-free plant production advancement, and for the knowledge expansion regarding the role of antioxidative enzymes in plant organogenesis.