The Ac124 protein is not essential for the propagation of Autographa californica multiple nucleopolyhedrovirus, but it is a viral pathogenicity factor
The Ac124 protein is not essential for the propagation of Autographa californica multiple nucleopolyhedrovirus, but it is a viral pathogenicity factor
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DOI：
10.1007/s00705-014-2277-y
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发表时间：
2015-01-01
影响因子：
2.7
通讯作者：
Chen, Xinwen
中科院分区：
文献类型：
--
作者：
Liang, Changyong;Lan, Dandan;Chen, Xinwen
AcMNPV的orf 124（ac 124）基因是鳞翅目核型多角体病毒属I组中高度保守的基因之一。到目前为止，它的功能仍然未知。在这项研究中，用表达ac 124-gfp融合体的病毒感染表明，Ac 124在整个感染过程中定位于细胞质。此外，ac 124基因敲除病毒的产生，以确定ac 124在杆状病毒生命周期中的作用。我们的研究结果表明，ac 124敲除AcMNPV可以产生感染性出芽病毒（BV）和闭塞体（OBs）一样，由野生病毒和ac 124修复病毒产生。这三种病毒在感染阶段具有相似的生长动力学。电镜下可见的核衣壳、包埋衍生病毒和OBs无显著差异。ac 124缺失突变体不能降低AcMNPV对S.甜菜碱的LD 50生物测定。ac 124缺失突变体对S.在LT 50生物测定中，甜菜夜蛾病毒比野生型病毒更强。总之，这些结果表明ac 124不是病毒复制所必需的，但它加速了被感染幼虫的死亡。
orf124 (ac124) of AcMNPV is one of the highly conserved unique genes in group I lepidopteran nucleopolyhedroviruses. So far, its function remains unknown. In this study, infection with a virus expressing an ac124-gfp fusion showed that Ac124 localized to the cytoplasm throughout the infection. In addition, an ac124 knockout virus was generated to determine the role of ac124 in the baculovirus life cycle. Our results showed that an ac124 knockout AcMNPV could produce infectious budded viruses (BVs) and occlusion bodies (OBs) like those produced by the wild virus and ac124 repair virus. These three viruses had similar growth kinetics during the infection phase. There was no significant difference in nucleocapsids, occlusion-derived viruses and OBs visualized by electron microscopy. The ac124 deletion mutant did not reduce AcMNPV infectivity for S. exigua in an LD50 bioassay. However, it took 20 h longer for the ac124 deletion mutant to kill S. exigua than wild-type virus in the LT50 bioassay. Altogether, these results demonstrate that ac124 is not required for viral replication, but it accelerates the killing of infected larvae.