Isolation of a non-traditionalsulfate reducing-bacteria Citrobacter freundii sp. and bioremoval of thalliumand sulfate
Isolation of a non-traditionalsulfate reducing-bacteria Citrobacter freundii sp. and bioremoval of thalliumand sulfate
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非传统硫酸盐还原细菌弗氏柠檬酸杆菌的分离。
DOI：
10.1016/j.ecoleng.2017.02.049
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发表时间：
2017
影响因子：
3.8
通讯作者：
张鸿郭
中科院分区：
文献类型：
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作者：
张鸿郭
从用于酸性矿山排水处理的上流式厌氧污泥床中分离出一种新型非传统硫酸盐还原菌（Sr 10），该污泥含有铊（Tl）和硫酸盐。对分离株 Sr 10 的部分 16S rRNA 基因序列的系统发育分析表明，它被鉴定为弗氏柠檬酸杆菌 (Citrobacter freundii) 物种。根据透射显微镜观察，Sr 10 视觉上呈杆状且具有周围鞭毛活动性极强。傅里叶变换红外光谱分析表明，该染色剂具有多种用于去除Tl和硫酸盐的官能团，包括羟基、羧基、酰胺基和磷酸基。在厌氧条件下，获得了该染色剂的最佳生长条件：温度35℃、初始pH值7.0。 Sr 10 能够同时去除Tl(I)和SO42−，去除效率分别高达99.60%和89.80%。该菌株可用于生物修复恢复过程中的 T1 和硫酸盐去除。
A novel non-traditional sulfate reducing bacterium (Sr 10) was isolated from an up-flow anaerobic sludge bed for acid mine drainage treatment which contains thallium (Tl) and sulfate. Phylogenetic analysis of partial 16S rRNA gene sequence of isolate Sr 10 revealed that it was identified to Citrobacter freundii species. Sr 10 was visually rod-shaped and very motile withperi-flagellum according to transmission microscopy. The Fourier transform infrared spectroscopy analysis indicated that the stains had various functional groups for Tl and sulfate removal, including hydroxyl, carboxyl, amide and phosphate. Under anaerobic conditions, the optimized growth conditions for the stain were obtained at temperature of 35 °C and initial pH value of 7.0. Sr 10 was able to remove both Tl(I) and SO42−simultaneously with the removal efficiency up to 99.60% and 89.80%, respectively. This strain might be used for Tl and sulfate removal in the process of bioremediation restoration.