The necessity of mitochondrial genome DNA for normal development of Dictyostelium cells
The necessity of mitochondrial genome DNA for normal development of Dictyostelium cells
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DOI：
10.1242/jcs.01140
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发表时间：
2004-07-01
影响因子：
4
通讯作者：
Maeda, Y
中科院分区：
文献类型：
--
作者：
Chida, J;Yamaguchi, H;Maeda, Y
最出乎意料的是，现在有越来越多的证据表明，线粒体在生长/分化转变，细胞类型确定，细胞运动和模式形成的调节机制中具有新颖和关键的功能。在这里，我们通过将盘基网柄藻Ax-2细胞暴露于ca. 30 μ/ml溴化乙锭（EtBr）的无菌生长培养基。重要的是，p（Delta）细胞表现出一系列迷人的行为：当它们被饥饿时，它们表现出明显的分化延迟，并在蛞蝓阶段停止发育，从而无法构建子实体。此外，细胞图案化和细胞类型比例被发现在源自p（Delta）细胞的段塞（称为p（Delta）段塞）中被极大地修改。也就是说，前茎分化显着增强p（德尔塔）蛞蝓，而前孢子分化显着抑制。此外，明确的前prestalk/后prepore模式在p（德尔塔）蛞蝓相当混乱，大概是因为两种类型的分化细胞之间的不完全分选。在测定趋光性之后，p（Delta）蛞蝓也表现出高度无序的朝向光源的运动。这些结果表明，mtDNA可能在网骨藻发育过程中的多种细胞过程中具有重要的功能。
Most unexpectedly, there is now increasing evidence that mitochondria have novel and crucial functions in the regulatory machinery of the growth/differentiation transition, cell-type determination, cellular movement and pattern formation. Here we created p(Delta) cells with a reduced amount (about 1/4) of mitochondrial DNA (mtDNA) from Dictyostelium discoideum Ax-2 cells, by exposing Ax-2 cells to ca. 30 mu/ml of ethidium bromide (EtBr) in axenic growth medium. Importantly, the p(Delta) cells exhibited a series of fascinating behaviors: when they were starved, they showed a marked delay of differentiation and stopped their development at the slug stage, thus failing to construct fruiting bodies. Moreover, cell patterning and cell-type proportioning were found to be greatly modified in slugs (referred to as p(Delta) slugs) derived from p(Delta) cells. That is, prestalk differentiation was significantly enhanced in p(Delta) slugs, while prespore differentiation was markedly inhibited. In addition, the clear anterior prestalk/posterior prespore pattern was considerably disturbed in p(Delta) slugs, presumably because of incomplete sorting between the two types of differentiated cells. After the assay of phototaxis, p(Delta) slugs also exhibited highly disordered movement towards the light source. Taken together, these results suggest that mtDNA might have important multiple functions in a variety of cellular processes during Dictyostelium development.