Tissue engineered hybrid tooth-bone constructs.
Tissue engineered hybrid tooth-bone constructs.
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DOI：
10.1016/j.ymeth.2008.09.004
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发表时间：
2009-02
期刊：
影响因子：
4.8
通讯作者：
Weibo Zhang;H. Abukawa;M. Troulis;L. Kaban;J. Vacanti;P. Yelick
中科院分区：
文献类型：
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作者：
Weibo Zhang;H. Abukawa;M. Troulis;L. Kaban;J. Vacanti;P. Yelick
由于颅面解剖结构的复杂性和组成组织的类型，颅面缺损的正确修复具有挑战性。同时协调多个组织再生的能力将使重建更有效，并可能降低发病率和改善结果。颅面复合体是独特的，因为牙齿的存在，除了皮肤，骨骼，软骨，肌肉，血管和神经组织，因为牙齿自然生长与颅面骨骼协调，我们的小组开发了一种自体的，牙齿-骨混合模型，以促进尤卡坦小型猪下颌骨缺损的修复。通过将牙芽细胞接种的支架与自体髂嵴骨髓来源的干细胞接种的支架组合来制备混合牙-骨结构，将其移植回同一小型猪的手术创建的下颌骨缺损中。在生长12周和20周后收获构建体。通过X射线、超高分辨率体积计算机断层扫描（VCT）、组织学、免疫组织化学分析和透射电子显微镜（TEM）对所得骨/牙齿结构进行评价。所观察到的由有组织的牙本质、釉质、牙髓、牙骨质、牙周韧带组成的小的牙齿样结构的形成，并且被再生的牙槽骨包围，表明在单个程序中牙齿和相关的牙槽骨再生的可行性。该模型为将来在人类中的临床应用提供了一种可访问的方法。
Proper rehabilitation of craniofacial defects is challenging because of the complexity of the anatomy and the component tissue types. The ability to simultaneously coordinate the regeneration of multiple tissues would make reconstruction more efficient and might reduce morbidity and improve outcomes. The craniofacial complex is unique because of the presence of teeth, in addition to skin, bone, cartilage, muscle, vascular, and neural tissues since teeth naturally grow in coordination with the craniofacial skeleton, our group developed an autologous, tooth–bone hybrid model to facilitate repair of mandibular defects in the Yucatan minipig. The hybrid tooth–bone construct was prepared by combining tooth bud cell-seeded scaffolds with autologous iliac crest bone marrow derived stem cell-seeded scaffolds, which were transplanted back into surgically created mandibular defects in the same minipig. The constructs were harvested after 12 and 20 weeks of growth. The resulting bone/tooth constructs were evaluated by X-ray, ultra high-resolution volume computed tomography (VCT), histological, immunohistochemical analyses, and transmission electron microscopy (TEM). The observed formation of small tooth-like structures consisting of organized dentin, enamel, pulp, cementum, periodontal ligament, and surrounded by regenerated alveolar bone, suggests the feasibility for regeneration of teeth and associated alveolar bone, in a single procedure. This model provides an accessible method for future clinical applications in humans.