Expression of estrogen receptor α and β mRNAs in prostate cancers treated with leuprorelin acetate
Expression of estrogen receptor α and β mRNAs in prostate cancers treated with leuprorelin acetate
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DOI：
10.1159/000020345
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发表时间：
2000-11-01
期刊：
影响因子：
23.4
通讯作者：
Usui, T
中科院分区：
文献类型：
--
作者：
Maruyama, S;Fujimoto, N;Usui, T
目的：新型雌激素受体 (ER) ER-β 的发现，为雌激素在前列腺中的作用提供了新的可能性。尽管据报道 ER-β 在大鼠前列腺中优先表达，但其在人类前列腺中的表达及其与癌症发展的关系尚未得到研究。因此本研究的目的是检测良性前列腺增生和前列腺癌中ER-α和ER-β的mRNA水平。方法：对在Radica I前列腺切除术中获得的15例前列腺癌样本进行检测。所有患者均维持雄激素戒断治疗至少3个月。采用竞争性 PCR 技术测量 ER-α 和 ER-β mRNA。结果：在所有检查的前列腺癌组织以及 PC3 和 LNCap 细胞中均检测到 ER-α 和 ER-β mRNA，尽管样本之间的水平有所不同。有趣的是，在淋巴结转移的情况下，两种类型均显着减少。然而，ER mRNA 水平与任何其他临床病理参数之间没有相关性。结论：(1) ER-α 和 ER-β mRNA 在前列腺癌中均表达，(2) ER mRNA 的表达可能与癌症进展无关，但可能与转移负相关，版权所有 (C) 2000 S. Karger AG，巴塞尔。
Objective: The discorvery of a novel estrogen receptor (ER), ER-beta, has given rise to new possibilities regarding estrogen's roles in the prostate. Although ER-beta is reported to be expressed preferentially in the rat prostate, its expression in the human prostate and relationship to cancer development has not been investigated. Thus the purpose of the study was to examine mRNA levels of ER-alpha and ER-beta in benign prostatic hyperplasia and prostate carcinoma,Methods: Samples of 15 prostate cancers obtained at rad ica I prostatectomy were examined. All the patients had been maintained on androgen withdrawal therapy for at least 3 months. ER-alpha and ER-beta mRNAs were measured with a competitive PCR technique.Results: Both ER-alpha and ER-beta mRNAs were detected in all of the prostate cancer tissues examined, as well as in PC3 and LNCap cells, although the levels varied among specimens. Interestingly, both types were significantly decreased in cases with lymph node metastasis. However, there was no correlation between ER mRNA levels and any other clinicopathological parameters,Conclusions: (1) Both ER-alpha and ER-beta mRNAs are expressed in prostate cancer and (2) expression of ER mRNA may not be related to cancer progression but may be negatively correlated with metastasis, Copyright (C) 2000 S. Karger AG, Basel.