The complete genome sequence of severe acute respiratory syndrome caronavirus strain HKU-39849 (HK-39)
The complete genome sequence of severe acute respiratory syndrome caronavirus strain HKU-39849 (HK-39)
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DOI：
10.1177/15353702-0322807-13
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发表时间：
2003-07-01
影响因子：
3.2
通讯作者：
Leung, FC
中科院分区：
文献类型：
--
作者：
Zeng, FY;Chan, CWM;Leung, FC
测定了香港严重急性呼吸综合征冠状病毒(SARS-CoV)HK-39株的全基因组序列(29.7kb)。基因组序列的系统发育分析表明，它是冠状病毒科的一个独特的成员。5‘RACE分析证实了至少6个亚基因组转录本的存在，它们都包含预测的基因间序列。5个开放阅读框(ORF1a、1b、S、M和N)与其他冠状病毒成员同源，另外3个未知ORF(X1、X2和X3)在所有已知的冠状病毒物种中都是不同的。同源ORF的最佳比对和计算机分析预测了推测基因上的特征结构和功能结构域。同源ORF的整体核苷酸保守性较低(
The complete genomic nucleotide sequence (29.7kb) of a Hong Kong severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV) strain HK-39 is determined. Phylogenetic analysis of the genomic sequence reveals it to be a distinct member of the Coronaviridae family. 5' RACE assay confirms the presence of at least six subgenomic transcripts all containing the predicted intergenic sequences. Five open reading frames (ORFs), namely ORF1a, 1b, S, M, and N, are found to be homologues to other CoV members, and three more unknown ORFs (X1, X2, and X3) are unparalleled in all other known CoV species. Optimal alignment and computer analysis of the homologous ORFs has predicted the characteristic structural and functional domains on the putative genes. The overall nucleotides conservation of the homologous ORFs is low (