Resurfacing potential of heterologous chondrocytes suspended in fibrin glue in large full-thickness defects of femoral articular cartilage: an experimental study in the goat
Resurfacing potential of heterologous chondrocytes suspended in fibrin glue in large full-thickness defects of femoral articular cartilage: an experimental study in the goat
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DOI：
10.1016/s0142-9612(97)00190-7
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发表时间：
1999-07-01
期刊：
影响因子：
14
通讯作者：
Veth, RPH
中科院分区：
文献类型：
--
作者：
van Susante, JLC;Buma, P;Veth, RPH
在32只成年山羊的股骨内侧髁上造成一个大的全层关节软骨缺损。将分离的兔软骨细胞悬浮在纤维蛋白胶中异种移植到缺损处。假手术山羊，其中只有一个标准化的缺陷，被用作对照。在3、8、13、26和52周后评估软骨修复的结果。对修复组织进行了肉眼、组织学和生化评价。结果表明，异种移植的兔软骨细胞在移植后存活，并保持其在纤维蛋白胶中产生基质的潜力，特别是如果它们位于非承重区域。在对异种移植软骨细胞的免疫反应方面，在两组中仅观察到轻微的滑膜炎体征，并且未发生移植细胞排斥。从3周开始，观察到纤维蛋白胶逐渐消退，随后被纤维组织替代。最初异种移植缺损的组织学表现出更好的趋势，软骨再生，然而，手术后52周，没有显着差异，可以检测到两组的修复组织宏观，组织学和生化评分。术后1年，新合成基质中II型胶原的量为75%。这项研究表明，分离的异源软骨细胞可用于关节软骨缺损的移植，然而，纤维蛋白胶不能为细胞提供足够的生物力学支持，以维持其作为三维支架的功能。(C)1999 Elsevier Science Ltd.保留所有权利。
A large full-thickness articular-cartilage defect was created in the medial femoral condyle of 32 adult goats. The defects were xenografted with isolated rabbit chondrocytes suspended in fibrin glue. Sham operated goats, where only a standardized defect was created, were used as controls. Results of cartilage repair were assessed after 3, 8, 13, 26 and 52 weeks. The repair tissue was evaluated macroscopically, histologically and biochemically. Results indicated that xenografted rabbit chondrocytes survived the transplantation and maintained their potential to produce matrix in fibrin glue, particularly if they were located in a non-weight-bearing area. In terms of an immunological reaction to xenografted chondrocytes, only mild signs of synovitis were observed in both groups and rejection of transplanted cells did not occur. From 3 weeks gradually progressive resolvement of the fibrin glue was observed with subsequent replacement by fibrous tissue. Initially xenografted defects histologically showed better tendency for cartilage regeneration, however, 52 weeks after surgery no significant differences could be detected in the repair tissue of both groups macroscopically, histologically and on biochemical scoring. The amount of collagen type II in the newly synthesized matrix was 75% 1 year after surgery. This study shows that isolated heterologous chondrocytes can be used for transplantation in articular cartilage defects, however, fibrin glue does not offer enough biomechanical support to the cells to maintain its function as a three-dimensional scaffold. (C) 1999 Elsevier Science Ltd. All rights reserved.