A novel suction electrode recording technique for monitoring circadian rhythms in single and multiunit discharge from brain slices
A novel suction electrode recording technique for monitoring circadian rhythms in single and multiunit discharge from brain slices
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DOI：
10.1016/j.jneumeth.2006.02.024
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发表时间：
2006-09-30
影响因子：
3
通讯作者：
Piggins, Hugh D.
中科院分区：
文献类型：
--
作者：
Brown, Timothy M.;Banks, Jon R.;Piggins, Hugh D.
在体外对自发神经元放电进行数天的研究提出了现有方法无法轻易解决的重大技术困难。在这里，我们描述了一种记录来自脑切片的生物电信号的新方法，使用通过负压稳定在记录界面的充满介质的玻璃微移液器。这种电极耐用、经济并且易于使用标准实验室设备构建。通过这些抽吸电极，我们在体外监测了急性准备的成年小鼠视交叉上核 (SCN) 切片中类似于 24 小时自发单单位和多单位放电的振荡长达 4 天，成功率非常高。神经元振荡表现出先前使用其他技术在 SCN 记录中确定的所有特征。重要的是，我们的电极组件能够比其他方法更长时间地监测精心准备的脑切片中的 SCN 活动。总之，我们的数据表明，抽吸电极可能为记录脑切片长期神经元活动的现有方法提供有利的替代方案。 (c) 2006 Elsevier B.V. 保留所有权利。
The study of spontaneous neuronal discharge for several days in vitro poses substantial technical difficulties not readily addressed by existing methodologies. Here we describe a novel method for recording bioelectrical signals from brain slices, using media-filled, glass micropipettes stabilized-at the recording interface by negative pressure. Such electrodes are durable, economical and easily constructed using standard laboratory equipment. Through these suction electrodes, we monitored similar to 24 h oscillations in spontaneous single and multiunit discharge from acutely prepared adult mouse suprachiasmatic nuclei (SCN) slices for up to 4 days in vitro, with very high success rates. Neuronal oscillations exhibited all the characteristics previously determined in SCN recordings using other techniques. Importantly, our electrode assembly enables SCN activity in acutely prepared brain slices to be monitored for substantially longer than with other methodologies. In summary, our data suggest that suction electrodes are likely to provide a favorable alternative to existing methods for recording long-term neuronal activity from brain slices. (c) 2006 Elsevier B.V. All rights reserved.