Distribution of mRNA for the facilitated urea transporter UT3 in the rat nervous system
Distribution of mRNA for the facilitated urea transporter UT3 in the rat nervous system
复制标题
DOI：
10.1007/s004290050152
复制
发表时间：
1998-05-01
期刊：
影响因子：
--
通讯作者：
Hediger, MA
中科院分区：
文献类型：
--
作者：
Berger, UV;Tsukaguchi, H;Hediger, MA
最近，编码大鼠尿素转运蛋白UT3的cDNA已从大鼠肾脏中克隆。在这里，我们描述的细胞定位的这种转运蛋白在大脑中检测到的非放射性原位杂交。UT3在整个中枢神经系统的星形胶质细胞以及小脑的Bergmann神经胶质细胞中表达。星形胶质细胞中的表达通过使用星形胶质细胞标记物GFAP的双重染色来验证。UT3 mRNA也强烈表达的室管膜细胞内衬脑室和视网膜中的米勒细胞。此外，UT3的表达在下丘和背根神经节，以及在垂体前叶的细胞中的神经元的亚群中检测到。其他类型的脑细胞，包括少突胶质细胞、小胶质细胞、伸长细胞、血管内皮细胞和脉络丛上皮细胞，都没有UT3 mRNA。北方印迹分析证实，在脑和背根神经节中的mRNA种类是相同的，并且培养的星形胶质细胞和C6细胞也表达UT3 mRNA。星形胶质细胞的UT3 mRNA表达在喹啉酸诱导的神经胶质增生中显著上调，这可能是神经胶质增生诱导的多胺形成过程中尿素水平增加的结果。我们认为星形胶质细胞中的UT3代表了一种控制脑中形成的尿素的机制，通过在整个星形胶质细胞网络中平衡尿素并将其引导到血管和CSF中进行处置。
Recently, the cDNA encoding the rat urea transporter UT3 has been cloned from rat kidney. Here we describe the cellular localization of this transporter in the brain as detected by non-radioactive in situ hybridization. UT3 is expressed in astrocytes throughout the central nervous system as well as in Bergmann glia in the cerebellum. The expression in astrocytes was verified by double staining using the astrocytic marker GFAP. UT3 mRNA is also strongly expressed by the ependymal cells lining the cerebral ventricles and by Muller cells in the retina. Furthermore, UT3 expression was detected in subgroups of neurons in the inferior colliculus and dorsal root ganglia, as well as in cells in the anterior pituitary gland. Other types of brain cells, including oligodendrocytes, microglia, tanycytes, endothelial cells of blood vessels, and epithelial cells in the choroid plexus were devoid of UT3 mRNA. Northern blot analysis confirmed that the mRNA species in the brain and in dorsal root ganglia are identical, and that cultured astrocytes and C6 cells also express the UT3 mRNA. UT3 mRNA expression by astrocytes is markedly upregulated in quinolinic acid-induced gliosis, possibly as a result of increased urea levels during gliosis induced polyamine formation. We propose that UT3 in astrocytes represents a mechanism to control urea formed in the brain by equilibrating it throughout the astrocyte network and guiding it to blood vessels and the CSF for disposal.