An individually coated near-infrared fluorescent protein as a safe and robust nanoprobe for in vivo imaging
An individually coated near-infrared fluorescent protein as a safe and robust nanoprobe for in vivo imaging
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单独涂层的近红外荧光蛋白作为体内成像的安全且稳定的纳米探针
DOI：
10.1039/c3nr02508j
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发表时间：
2013
期刊：
影响因子：
6.7
通讯作者：
Cao Aoneng
中科院分区：
文献类型：
--
作者：
Yang Yu;Xiang Kun;Yang Yi-Xin;Wang Yan-Wen;Zhang Xin;Cui Yangdong;Wang Haifang;Zhu Qing-Qing;Fan Liqiang;Liu Yuanfang;Cao Aoneng
荧光探针的安全性是进行体内荧光成像的先决条件。在所有的荧光探针中，荧光蛋白可能是最安全的一种，在基因水平上被广泛应用于生物科学。但由于蛋白质的不稳定性，FPs尚未以纯化形式在体内使用。在这里，我们分别用二氧化硅纳米壳包裹近红外（NIR） FPs (NIRFPs)，从而得到NIRFP@silica，这是最安全，最亮的近红外荧光纳米探针之一，量子产率为0.33，用于体内成像。二氧化硅外壳不仅可以防止NIRFPs的变性和代谢消化，还可以提高涂层NIRFPs的量子产率和光稳定性。当通过尾静脉注射时，NIRFP@silica NPs可以分布在小鼠全身各处，并在24小时内通过尿液有效消除，显示了其作为安全可靠的全身成像近红外荧光探针的潜在应用前景。
A prerequisite for in vivo fluorescence imaging is the safety of fluorescent probes. Among all fluorescent probes, fluorescent proteins (FPs) might be the safest ones, which have been widely used in biological sciences at the gene level. But FPs have not been used in vivo in the purified form yet due to the instability of proteins. Here, we individually coat near-infrared (NIR) FPs (NIRFPs) with a silica nanoshell, resulting in NIRFP@silica, one of the safest and brightest NIR fluorescent nanoprobes with a quantum yield of 0.33 for in vivo imaging. The silica shell not only protects NIRFPs from denaturation and metabolic digestion, but also enhances the quantum yield and photostability of the coated NIRFPs. When injected via the tail vein, NIRFP@silica NPs can distribute all over the mouse body, and then can be efficiently eliminated through urine in 24 h, demonstrating its potential applications as a safe and robust NIR fluorescence probe for whole body imaging.