Heterogeneous nuclear ribonucleoprotein-H plays a suppressive role in visceral myogenesis
Heterogeneous nuclear ribonucleoprotein-H plays a suppressive role in visceral myogenesis
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DOI：
10.1016/s0925-4773(01)00377-x
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发表时间：
2001-06-01
影响因子：
2.6
通讯作者：
Schuger, L
中科院分区：
文献类型：
--
作者：
Liu, JH;Beqaj, S;Schuger, L
小鼠胚胎间充质细胞在培养物中伸展/伸长时经历自发平滑肌（SM）分化（Relan等人，J. Cell Biol.147（1999）1341; Yang等人，Development 125（1998）2621; Yang等人，Development 126（1999）3027）。使用这些细胞，我们产生了一个消减cDNA文库，以确定潜在的SM肌生成抑制。差异表达的基因之一是异质核核糖核蛋白-H（hnRNP-H），其参与前体mRNA的选择性剪接，hnRNP-H在SM分化开始前的间充质细胞中高表达，但在SM肌发生的间充质细胞中其表达迅速下降。在体内，hnRNP-H表达的下降仅限于内脏SM细胞。反义寡核苷酸和反义RNA被用来抑制hnRNP-H合成在SM分化间充质细胞和胚胎肺外植体。hnRNP-H水平的降低导致SM特异性基因表达的上调和肺外植体中支气管SM发育的增加。细胞培养物中hnRNP-H过表达具有相反的效果。因此，这些研究表明hnRNP-H在控制内脏肌发生中的新作用。(C)2001爱思唯尔科学爱尔兰有限公司保留所有权利。
Mouse embryonic mesenchymal cells undergo spontaneous smooth muscle (SM) differentiation upon spreading/elongation in culture (Relan et al., J. Cell Biol. 147 (1999) 1341; Yang et al., Development 125 (1998) 2621; Yang et al., Development 126 (1999) 3027). Using these cells we generated a subtracted cDNA library to identify potential suppressors of SM myogenesis. One of the differentially expressed genes was heterogeneous nuclear ribonucleoprotein-H (hnRNP-H), which is involved in pre-mRNA alternative splicing, hnRNP-H was highly expressed in mesenchymal cells prior to the onset of SM differentiation, but its expression rapidly decreased in mesenchymal cells undergoing SM myogenesis. In vivo, the drop in hnRNP-H expression was restricted to visceral SM cells. Antisense oligodeoxynucleotide and antisense RNA were used to inhibit hnRNP-H synthesis in SM-differentiating mesenchymal cells and in embryonic lung explants. A decrease in hnRNP-H levels resulted in upregulation of SM-specific gene expression and increased bronchial SM development in lung explants. hnRNP-H overexpression in cell cultures had the opposite effect. These studies, therefore, indicate a novel role for hnRNP-H in the control of visceral myogenesis. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.