An ELISA for measuring serum antibodies to Actinobacillus actinomycetemcomitans.
An ELISA for measuring serum antibodies to Actinobacillus actinomycetemcomitans.
复制标题
用于测量伴放线放线杆菌血清抗体的 ELISA。
DOI：
10.1111/j.1600-0765.1980.tb00321.x
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发表时间：
1980
影响因子：
3.5
通讯作者：
Smith,DJ
中科院分区：
文献类型：
--
作者：
Ebersole,JL;Frey,DE;Taubman,MA;Smith,DJ
本文描述了间接酶联免疫吸附试验（ELISA）的适应性，用于测定针对完整的放线菌杆菌和声波细菌的同种型特异性抗体。对9名确诊或未确诊牙周病的患者的血清进行了微生物IgG抗体检测。研究了测定条件，并与细菌凝集法、间接免疫荧光法和被动血凝法进行了比较。在将血清分为高、中、低抗体活性的分析中，注意到正相关。然而，基于在检测中产生阳性反应的血清的最高稀释度，改进的ELISA比微凝集、间接免疫荧光或被动血凝技术的敏感性高5-50倍。ELISA是一种使用少量生物材料测定同种型特异性抗体水平的灵敏测定方法。该试验可提供一种快速筛选大量生物样品的完整微生物抗体的方法。
An adaptation of the indirect enzyme‐linked immunosorbent assay (ELISA) is described for the determination of isotype specific antibodies directed to intactActinobacillus actinomycetemcomitansand bacterial sonicates. Sera from nine individuals, with or without diagnosed periodontal disease, have been examined for IgG antibodies to the microorganism. The conditions for the assay have been studied and the ELISA has been compared to bacterial agglutination, indirect immunofluorescence or passive hemagglutination. A positive correlation is noted among the analyses in separating the sera into high, moderate and low antibody activities. However, based upon the highest dilution of the sera that gave positive reactions in the assays, the modified ELISA is 5–50 fold more sensitive than either the microagglutination, indirect immunofluorescence or passive hemagglutination techniques as performed. The ELISA is a sensitive assay that uses small quantities of biological materials to determine isotype specific antibody levels. This assay can provide a means for rapid screening of large numbers of biological samples for antibodies to intact microorganisms.