Enhancement of Oral Tolerance Induction in DO11.10 Mice by Lactobacillus gasseri OLL2809 via Increase of Effector Regulatory T Cells.
Enhancement of Oral Tolerance Induction in DO11.10 Mice by Lactobacillus gasseri OLL2809 via Increase of Effector Regulatory T Cells.
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DOI：
10.1371/journal.pone.0158643
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Totsuka M
中科院分区：
文献类型：
--
作者：
Aoki-Yoshida A;Yamada K;Hachimura S;Sashihara T;Ikegami S;Shimizu M;Totsuka M
食物过敏是婴幼儿的一个严重问题。诱导抗原特异性口服耐受是一种治疗策略。通过饮食诱导提高口服耐受性是预防婴儿食物过敏的一种有前景的策略。因此，在这项研究中，我们评估了益生菌发酵乳杆菌OLL2809(LG2809)对小鼠口服耐受性诱导的影响。通过检测单用卵白蛋白(OVA)或OVA加LG2809对DO11.10小鼠脾CD4+T细胞增殖和IL-2产生水平的影响来评价口服耐受的程度。口服LG2809显著降低了OVA喂养小鼠的CD4+T细胞的增殖率和IL-2的产生。LG2809提高了CD4+T细胞的比例，产生高水平的IL-10，并具有很强的抑制活性。此外，LG2809还增加了小肠固有层中浆细胞样树突状细胞(PDC)的比例。用LG2809喂养的BALB/c小鼠的LP细胞作为DO11.10小鼠幼稚的CD4+T细胞的抗原提呈细胞，与未处理的小鼠相比，诱导产生更高的IL-10和更强的抑制活性。这些结果表明，口服LG2809增加了LP中pDC的数量，从而通过增加效应性调节性T细胞的比例而增强了口服耐受诱导。因此，LG2809可以作为一种有效的免疫调节剂，通过促进口服耐受性来预防食物过敏。
Food allergy is a serious problem for infants and young children. Induction of antigen-specific oral tolerance is one therapeutic strategy. Enhancement of oral tolerance induction by diet is a promising strategy to prevent food allergy in infants. Thus, in this study, we evaluate the effect of probiotic Lactobacillus gasseri OLL2809 (LG2809) on oral tolerance induction in a mouse model. The degree of oral tolerance induction was evaluated by measuring the proliferation and level of IL-2 production of splenic CD4+ T cells from DO11.10 mice fed ovalbumin (OVA) alone or OVA with LG2809. Oral administration of LG2809 significantly decreased the rate of proliferation and IL-2 production by CD4+ T cells from OVA-fed mice. LG2809 increased a ratio of CD4+ T-cell population, producing high levels of IL-10 and having strong suppressive activity. Moreover, LG2809 increased a ratio of plasmacytoid dendritic cells (pDCs) among the lamina propria (LP) in small intestine. When used as antigen presenting cells to naïve CD4+ T cells from DO11.10 mice, LP cells from BALB/c mice fed LG2809 induced higher IL-10 production and stronger suppressive activity than those from non-treated mice. These results suggest that oral administration of LG2809 increases the population of pDCs in the LP, resulting in the enhancement of oral tolerance induction by increasing the ratio of effector regulatory T cells. LG2809 could, therefore, act as a potent immunomodulator to prevent food allergies by promoting oral tolerance.