Structural insight into pre-B cell receptor function
Structural insight into pre-B cell receptor function
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DOI：
10.1126/science.1139412
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发表时间：
2007-04-13
期刊：
影响因子：
56.9
通讯作者：
Garcia, K. Christopher
中科院分区：
文献类型：
--
作者：
Bankovich, Alexander J.;Raunser, Stefan;Garcia, K. Christopher
B细胞前受体（pre-BCR）在B细胞发育过程中起检查点作用。在人bcr前fab样片段的2.7埃结构中，由抗体重链（HC）与替代轻链配对组成，VpreB和lambda 5的“独特区域”取代了抗体轻链的互补决定区3 （CDR3）环，似乎“探测”了HC CDR3，可能影响抗体库的选择。生化分析表明，pre-BCR识别抗原的能力受损，再加上电子显微镜下bcr前二聚体的可视化，表明配体非依赖性寡聚化可能是信号机制。
The pre-B cell receptor (pre-BCR) serves as a checkpoint in B cell development. In the 2.7 angstrom structure of a human pre-BCR Fab-like fragment, consisting of an antibody heavy chain (HC) paired with the surrogate light chain, the "unique regions" of VpreB and lambda 5 replace the complementarity-determining region 3 (CDR3) loop of an antibody light chain and appear to "probe" the HC CDR3, potentially influencing the selection of the antibody repertoire. Biochemical analysis indicates that the pre-BCR is impaired in its ability to recognize antigen, which, together with electron microscopic visualization of a pre-BCR dimer, suggests ligand-independent oligomerization as the likely signaling mechanism.