Fiber orientation in a whole mouse heart reconstructed by laboratory phase-contrast micro-CT
Fiber orientation in a whole mouse heart reconstructed by laboratory phase-contrast micro-CT
复制标题
通过实验室相差显微 CT 重建整个小鼠心脏的纤维方向
DOI：
10.1117/1.jmi.7.2.023501
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发表时间：
2019
影响因子：
2.4
通讯作者：
T. Salditt
中科院分区：
文献类型：
--
作者：
Marius Reichardt;M. Töpperwien;Amara Khan;F. Alves;T. Salditt
抽象。目的：我们提出了一种相位对比X射线断层扫描研究野生型C57BL/6小鼠心脏作为一种无损的方法，显微解剖的规模上的整个切除器官。基于部分相干性在自制的相位对比μ-CT装置安装在液态金属射流源，我们利用相位恢复，从而实现上级图像质量的心脏组织，几乎可以媲美以前的同步辐射数据在整个器官的规模。方法：在我们的工作中，已经探索了不同的包埋方法和基于重金属的染色剂。从层析重建，定量的结构参数描述的三维（3-D）架构已经推导出两种不同的纤维跟踪算法。第一种算法是基于重建的电子密度的局部梯度。通过对小子体积的局部结构张量进行主成分分析，可以确定体积内部的主导方向。除了这种方法，这是已经很好地建立了心脏组织，我们已经实施和测试的算法，是基于一个本地的3-D傅立叶变换。结果如下：我们表明，样品制备的选择影响组织的3-D结构，不仅在对比度方面，而且在结构保存方面。用溶剂蒸发法制备的心脏用于比较两种算法。两种方法的结构取向的结果非常相似。除了确定的纤维方向，细丝排列的程度和局部厚度的单肌纤维束，获得使用傅立叶为基础的方法。结论：相位衬度X射线断层扫描允许以低于10 μ m的各向同性分辨率研究心脏组织的结构。事实上，这是可能的紧凑的实验室仪器开辟了新的机会，筛选样品和优化样品制备，也之前同步加速器光束时间。此外，来自结构分析的结果可以帮助理解心血管疾病，或者可以用于改进心脏的计算模型。
Abstract. Purpose: We present a phase-contrast x-ray tomography study of wild type C57BL/6 mouse hearts as a nondestructive approach to the microanatomy on the scale of the entire excised organ. Based on the partial coherence at a home-built phase-contrast μ-CT setup installed at a liquid metal jet source, we exploit phase retrieval and hence achieve superior image quality for heart tissue, almost comparable to previous synchrotron data on the whole organ scale. Approach: In our work, different embedding methods and heavy metal-based stains have been explored. From the tomographic reconstructions, quantitative structural parameters describing the three-dimensional (3-D) architecture have been derived by two different fiber tracking algorithms. The first algorithm is based on the local gradient of the reconstructed electron density. By performing a principal component analysis on the local structure-tensor of small subvolumes, the dominant direction inside the volume can be determined. In addition to this approach, which is already well established for heart tissue, we have implemented and tested an algorithm that is based on a local 3-D Fourier transform. Results: We showed that the choice of sample preparation influences the 3-D structure of the tissue, not only in terms of contrast but also with respect to the structural preservation. A heart prepared with the evaporation-of-solvent method was used to compare both algorithms. The results of structural orientation were very similar for both approaches. In addition to the determination of the fiber orientation, the degree of filament alignment and local thickness of single muscle fiber bundles were obtained using the Fourier-based approach. Conclusions: Phase-contrast x-ray tomography allows for investigating the structure of heart tissue with an isotropic resolution below 10 μm. The fact that this is possible with compact laboratory instrumentation opens up new opportunities for screening samples and optimizing sample preparation, also prior to synchrotron beamtimes. Further, results from the structural analysis can help in understanding cardiovascular diseases or can be used to improve computational models of the heart.
影响因子：
64.8
作者：
Christoph, J.;Chebbok, M.;Luther, S.
通讯作者：
Luther, S.
影响因子：
4.6
作者：
Töpperwien M;Krenkel M;Vincenz D;Stöber F;Oelschlegel AM;Goldschmidt J;Salditt T
通讯作者：
Salditt T