Comparison of two methods for enumerating circulating tumor cells in carcinoma patients
Comparison of two methods for enumerating circulating tumor cells in carcinoma patients
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DOI：
10.1002/cyto.b.20065
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发表时间：
2005-11-01
影响因子：
3.4
通讯作者：
Bauernhofer, T
中科院分区：
文献类型：
--
作者：
Balic, M;Dandachi, N;Bauernhofer, T
背景：监测接受新型化合物治疗的癌症患者血液中的循环肿瘤细胞（CTC）可能是治疗效果的衡量标准。在临床使用之前，需要一种可靠的方法来计数 CTC。我们比较了两种 CTC 计数方法：Onko-Quick 和 CellSearch 系统。方法：我们从 15 名健康捐献者和 61 名转移癌患者身上抽取 22.5 毫升血液到三个 CellSave 试管中。合并后，用 OncoQuick 处理 15 ml，用 CellSearch 处理 7.5 ml。 结果：两种方法均未在健康供体中发现 CTC。使用 OncoQuick 的 61 名患者中有 14 名 (23%) 检测到至少一种 CTC，使用 CellSearch 的 61 名患者中有 33 名 (54%) 检测到至少一种 CTC (P < 0.0001)。 CellSearch 检测到的 CTC 数量（平均 20 个 CTC/7.5 ml 血液）高于 OncoQuick（3 个 CTC/7.5 ml；P < 0.0001）。 结论：CellSearch 系统是一种更准确、更灵敏的 CTC 计数方法。需要进一步的研究来评估 CellSearch 系统的 CTC 计数作为评估新型抗癌药物疗效的监测工具。 (c) 2005 年国际分析细胞学学会。
Background: Monitoring of circulating tumor cells (CTCs) in blood of carcinoma patients treated with novel compounds may be a measurement of treatment effectiveness. Before it can be used clinically, a reliably method is needed to enumerate CTCs. We compared two methods for CTC enumeration, Onko-Quick and the CellSearch system.Methods: We drew 22.5 ml of blood into three CellSave tubes from 15 healthy donors and 61 patients with metastatic carcinoma. After pooling, 15 ml was processed with OncoQuick and 7.5 ml with CellSearch.Results: With both methods no CTCs were found in healthy donors. At least one CTC was detected in 14 of 61 patients (23%) with OncoQuick and 33 of 61 patients (54%) with CellSearch (P < 0.0001). The number of CTCs detected was larger for CellSearch (mean 20 CTCs/7.5 ml of blood) than for OncoQuick (3 CTCs/7.5 ml; P < 0.0001).Conclusion: The CellSearch system is a more accurate and sensitive method to enumerate CTCs. Further studies are warranted to evaluate CTC enumeration by the CellSearch system as a monitoring tool for the evaluation of the efficacy of novel anticancer agents. (c) 2005 international Society for Analytical Cytology.