Piperine inhibits LPS induced expression of inflammatory mediators in RAW 264.7 cells
Piperine inhibits LPS induced expression of inflammatory mediators in RAW 264.7 cells
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DOI：
10.1016/j.cellimm.2013.09.001
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发表时间：
2013-09-01
影响因子：
4.3
通讯作者：
Peng, Lei
中科院分区：
文献类型：
--
作者：
Ying, Xiaozhou;Yu, Kehe;Peng, Lei
本研究旨在探讨胡椒碱对RAW264.7细胞脂多糖（LPS）炎症反应的影响及其信号转导途径。分别以10、50、100 μ g/ml胡椒碱预处理RAW264.7细胞，然后用LPS （1 μ g/ml）刺激24h。我们发现胡椒碱能抑制LPS诱导的前列腺素E2 （PGE2）和一氧化氮（NO）的产生。胡椒碱显著降低lps刺激的RAW264.7细胞中肿瘤坏死因子- α (TNF - α)、诱导型NO合成酶（iNOS）和COX-2的基因表达和产生。胡椒碱通过抑制抑制因子κ B蛋白（I κ B）的降解和nf - κ B p65亚基从细胞质到细胞核的易位，抑制lps介导的核因子κ B （nf - κ B）的活化。结果表明胡椒碱对RAW264.7细胞具有抗炎作用；这表明胡椒碱可能是一种治疗炎症的潜在药物。(C) 2013爱思唯尔公司版权所有。
The aim of the present study was to investigate the effects of piperine on the inflammatory responses to lipopolysaccharide (LPS) in RAW264.7 cells and the signal transduction pathways involved. RAW264.7 cells were pretreated with piperine at 10, 50 or 100 mu g/ml and subsequently stimulated with LPS (1 mu g/ml) for 24 h. We found that piperine inhibited the production of prostaglandin E2 (PGE2) and nitric oxide (NO) induced by LPS. Piperine significantly decreased LPS-stimulated gene expression and production of tumor necrosis factor-alpha (TNF alpha), inducible NO synthase (iNOS) and COX-2 in RAW264.7 cells. Piperine inhibited the LPS-mediated activation of nuclear factor-kappa B (NF-kappaB) by suppressing the degradation of inhibitor-kappa B proteins (I kappa B) and the translocations of p65 subunit of NF-kappa B from the cytosol to the nucleus. Our results demonstrate the anti-inflammatory activity of piperine in RAW264.7 cells; suggesting that piperine may be a potential agent in the treatment of inflammation. (C) 2013 Elsevier Inc. All rights reserved.